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Characterisation of bacteria isolated from stingless bee, Heterotrigona itama, honey, bee bread and propolis

Bacteria are present in stingless bee nest products. However, detailed information on their characteristics is scarce. Thus, this study aims to investigate the characteristics of bacterial species isolated from Malaysian stingless bee, Heterotrigona itama, nest products. Honey, bee bread and propoli...

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Main Authors: Ngalimat, Mohamad Syazwan, Raja Abd Rahman, Raja Noor Zaliha, Yusof, Mohd Termizi, Amir Hamzah, Amir Syahir, Sabri, Suriana
פורמט: Article
שפה:English
יצא לאור: PeerJ 2019
גישה מקוונת:http://psasir.upm.edu.my/id/eprint/80896/1/BACT.pdf
תגים: הוספת תג
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spelling oai:psasir.upm.edu.my:80896 http://psasir.upm.edu.my/id/eprint/80896/ Characterisation of bacteria isolated from stingless bee, Heterotrigona itama, honey, bee bread and propolis Ngalimat, Mohamad Syazwan Raja Abd Rahman, Raja Noor Zaliha Yusof, Mohd Termizi Amir Hamzah, Amir Syahir Sabri, Suriana Bacteria are present in stingless bee nest products. However, detailed information on their characteristics is scarce. Thus, this study aims to investigate the characteristics of bacterial species isolated from Malaysian stingless bee, Heterotrigona itama, nest products. Honey, bee bread and propolis were collected aseptically from four geographical localities of Malaysia. Total plate count (TPC), bacterial identification, phenotypic profile and enzymatic and antibacterial activities were studied. The results indicated that the number of TPC varies from one location to another. A total of 41 different bacterial isolates from the phyla Firmicutes, Proteobacteria and Actinobacteria were identified. Bacillus species were the major bacteria found. Therein, Bacillus cereus was the most frequently isolated species followed by Bacillus aryabhattai, Bacillus oleronius, Bacillus stratosphericus, Bacillus altitudinis, Bacillus amyloliquefaciens, Bacillus nealsonii, Bacillus toyonensis, Bacillus subtilis, Bacillus safensis, Bacillus pseudomycoides, Enterobacter asburiae, Enterobacter cloacae, Pantoea dispersa and Streptomyces kunmingensis. Phenotypic profile of 15 bacterial isolates using GEN III MicroPlate™ system revealed most of the isolates as capable to utilise carbohydrates as well as amino acids and carboxylic acids and derivatives. Proteolytic, lipolytic and cellulolytic activities as determined by enzymatic assays were detected in Bacillus stratosphericus PD6, Bacillus amyloliquefaciens PD9, Bacillus subtilis BD3 and Bacillus safensis BD9. Bacillus amyloliquefaciens PD9 showed broad-spectrum of antimicrobial activity against Gram-positive and Gram-negative bacteria in vitro. The multienzymes and antimicrobial activities exhibited by the bacterial isolates from H. itama nest products could provide potential sources of enzymes and antimicrobial compounds for biotechnological applications. PeerJ 2019 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/80896/1/BACT.pdf Ngalimat, Mohamad Syazwan and Raja Abd Rahman, Raja Noor Zaliha and Yusof, Mohd Termizi and Amir Hamzah, Amir Syahir and Sabri, Suriana (2019) Characterisation of bacteria isolated from stingless bee, Heterotrigona itama, honey, bee bread and propolis. PeerJ, 7. pp. 1-20. ISSN 2167-8359; ESSN: 2167-9843 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6708576/pdf/peerj-07-7478.pdf 10.7717/peerj.7478
institution UPM IR
collection UPM IR
language English
description Bacteria are present in stingless bee nest products. However, detailed information on their characteristics is scarce. Thus, this study aims to investigate the characteristics of bacterial species isolated from Malaysian stingless bee, Heterotrigona itama, nest products. Honey, bee bread and propolis were collected aseptically from four geographical localities of Malaysia. Total plate count (TPC), bacterial identification, phenotypic profile and enzymatic and antibacterial activities were studied. The results indicated that the number of TPC varies from one location to another. A total of 41 different bacterial isolates from the phyla Firmicutes, Proteobacteria and Actinobacteria were identified. Bacillus species were the major bacteria found. Therein, Bacillus cereus was the most frequently isolated species followed by Bacillus aryabhattai, Bacillus oleronius, Bacillus stratosphericus, Bacillus altitudinis, Bacillus amyloliquefaciens, Bacillus nealsonii, Bacillus toyonensis, Bacillus subtilis, Bacillus safensis, Bacillus pseudomycoides, Enterobacter asburiae, Enterobacter cloacae, Pantoea dispersa and Streptomyces kunmingensis. Phenotypic profile of 15 bacterial isolates using GEN III MicroPlate™ system revealed most of the isolates as capable to utilise carbohydrates as well as amino acids and carboxylic acids and derivatives. Proteolytic, lipolytic and cellulolytic activities as determined by enzymatic assays were detected in Bacillus stratosphericus PD6, Bacillus amyloliquefaciens PD9, Bacillus subtilis BD3 and Bacillus safensis BD9. Bacillus amyloliquefaciens PD9 showed broad-spectrum of antimicrobial activity against Gram-positive and Gram-negative bacteria in vitro. The multienzymes and antimicrobial activities exhibited by the bacterial isolates from H. itama nest products could provide potential sources of enzymes and antimicrobial compounds for biotechnological applications.
format Article
author Ngalimat, Mohamad Syazwan
Raja Abd Rahman, Raja Noor Zaliha
Yusof, Mohd Termizi
Amir Hamzah, Amir Syahir
Sabri, Suriana
spellingShingle Ngalimat, Mohamad Syazwan
Raja Abd Rahman, Raja Noor Zaliha
Yusof, Mohd Termizi
Amir Hamzah, Amir Syahir
Sabri, Suriana
Characterisation of bacteria isolated from stingless bee, Heterotrigona itama, honey, bee bread and propolis
author_facet Ngalimat, Mohamad Syazwan
Raja Abd Rahman, Raja Noor Zaliha
Yusof, Mohd Termizi
Amir Hamzah, Amir Syahir
Sabri, Suriana
author_sort Ngalimat, Mohamad Syazwan
title Characterisation of bacteria isolated from stingless bee, Heterotrigona itama, honey, bee bread and propolis
title_short Characterisation of bacteria isolated from stingless bee, Heterotrigona itama, honey, bee bread and propolis
title_full Characterisation of bacteria isolated from stingless bee, Heterotrigona itama, honey, bee bread and propolis
title_fullStr Characterisation of bacteria isolated from stingless bee, Heterotrigona itama, honey, bee bread and propolis
title_full_unstemmed Characterisation of bacteria isolated from stingless bee, Heterotrigona itama, honey, bee bread and propolis
title_sort characterisation of bacteria isolated from stingless bee, heterotrigona itama, honey, bee bread and propolis
publisher PeerJ
publishDate 2019
url http://psasir.upm.edu.my/id/eprint/80896/1/BACT.pdf
_version_ 1819299696224501760
score 13.4562235

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