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Phytochemical evaluation, embryotoxicity and teratogenic effects of Curcuma longa extract on zebrafish (Danio rerio)

Curcuma longa L. is a rhizome plant often used as traditional medicinal preparations in Southeast Asia. The dried powder is commonly known as cure-all herbal medicine with a wider spectrum of pharmaceutical activities. In spite of the widely reported therapeutic applications of C. longa, research on...

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Asıl Yazarlar: Adekoya, Alafiatayo Akinola, Lai, Kok Song, Ahmad, Syahida, Mahmood, Maziah, Shaharuddin, Noor Azmi
Materyal Türü: Makale
Dil:English
Baskı/Yayın Bilgisi: Hindawi Limited 2019
Online Erişim:http://psasir.upm.edu.my/id/eprint/81739/1/Phytochemical%20evaluation%2C%20embryotoxicity.pdf
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spelling oai:psasir.upm.edu.my:81739 http://psasir.upm.edu.my/id/eprint/81739/ Phytochemical evaluation, embryotoxicity and teratogenic effects of Curcuma longa extract on zebrafish (Danio rerio) Adekoya, Alafiatayo Akinola Lai, Kok Song Ahmad, Syahida Mahmood, Maziah Shaharuddin, Noor Azmi Curcuma longa L. is a rhizome plant often used as traditional medicinal preparations in Southeast Asia. The dried powder is commonly known as cure-all herbal medicine with a wider spectrum of pharmaceutical activities. In spite of the widely reported therapeutic applications of C. longa, research on its safety and teratogenic effects on zebrafish embryos and larvae is still limited. Hence, this research aimed to assess the toxicity of C. longa extract on zebrafish. Using a reflux flask, methanol extract of C. longa was extracted and the identification and quantification of total flavonoids were carried out with HPLC. Twelve fertilized embryos were selected to test the embryotoxicity and teratogenicity at different concentration points. The embryos were exposed to the extract in the E3M medium while the control was only exposed to E3M and different developmental endpoints were recorded with the therapeutic index calculated using the ratio of LC50/EC50. C. longa extract was detected to be highly rich in flavonoids with catechin, epicatechin, and naringenin as the 3 most abundant with concentrations of 3,531.34, 688.70, and 523.83μg/mL, respectively. The toxicity effects were discovered to be dose-dependent at dosage above 62.50μg/mL, while, at 125.0μg/mL, mortality of embryos was observed and physical body deformities of larvae were recorded among the hatched embryos at higher concentrations. Teratogenic effects of the extract was severe at higher concentrations producing physical body deformities such as kink tail, bend trunk, and enlarged yolk sac edema. Finally, the therapeutic index (TI) values calculated were approximately the same for different concentration points tested. Overall, the result revealed that plants having therapeutic potential could also pose threats when consumed at higher doses especially on the embryos. Therefore, detailed toxicity analysis should be carried out on medicinal plants to ascertain their safety on the embryos and its development. Hindawi Limited 2019-03-05 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/81739/1/Phytochemical%20evaluation%2C%20embryotoxicity.pdf Adekoya, Alafiatayo Akinola and Lai, Kok Song and Ahmad, Syahida and Mahmood, Maziah and Shaharuddin, Noor Azmi (2019) Phytochemical evaluation, embryotoxicity and teratogenic effects of Curcuma longa extract on zebrafish (Danio rerio). Evidence-based Complementary and Alternative Medicine, 2019. art. no. 3807207. pp. 1-11. ISSN 1741-427X; ESSN: 1741-4288 https://www.hindawi.com/journals/ecam/2019/3807207/ 10.1155/2019/3807207
institution UPM IR
collection UPM IR
language English
description Curcuma longa L. is a rhizome plant often used as traditional medicinal preparations in Southeast Asia. The dried powder is commonly known as cure-all herbal medicine with a wider spectrum of pharmaceutical activities. In spite of the widely reported therapeutic applications of C. longa, research on its safety and teratogenic effects on zebrafish embryos and larvae is still limited. Hence, this research aimed to assess the toxicity of C. longa extract on zebrafish. Using a reflux flask, methanol extract of C. longa was extracted and the identification and quantification of total flavonoids were carried out with HPLC. Twelve fertilized embryos were selected to test the embryotoxicity and teratogenicity at different concentration points. The embryos were exposed to the extract in the E3M medium while the control was only exposed to E3M and different developmental endpoints were recorded with the therapeutic index calculated using the ratio of LC50/EC50. C. longa extract was detected to be highly rich in flavonoids with catechin, epicatechin, and naringenin as the 3 most abundant with concentrations of 3,531.34, 688.70, and 523.83μg/mL, respectively. The toxicity effects were discovered to be dose-dependent at dosage above 62.50μg/mL, while, at 125.0μg/mL, mortality of embryos was observed and physical body deformities of larvae were recorded among the hatched embryos at higher concentrations. Teratogenic effects of the extract was severe at higher concentrations producing physical body deformities such as kink tail, bend trunk, and enlarged yolk sac edema. Finally, the therapeutic index (TI) values calculated were approximately the same for different concentration points tested. Overall, the result revealed that plants having therapeutic potential could also pose threats when consumed at higher doses especially on the embryos. Therefore, detailed toxicity analysis should be carried out on medicinal plants to ascertain their safety on the embryos and its development.
format Article
author Adekoya, Alafiatayo Akinola
Lai, Kok Song
Ahmad, Syahida
Mahmood, Maziah
Shaharuddin, Noor Azmi
spellingShingle Adekoya, Alafiatayo Akinola
Lai, Kok Song
Ahmad, Syahida
Mahmood, Maziah
Shaharuddin, Noor Azmi
Phytochemical evaluation, embryotoxicity and teratogenic effects of Curcuma longa extract on zebrafish (Danio rerio)
author_facet Adekoya, Alafiatayo Akinola
Lai, Kok Song
Ahmad, Syahida
Mahmood, Maziah
Shaharuddin, Noor Azmi
author_sort Adekoya, Alafiatayo Akinola
title Phytochemical evaluation, embryotoxicity and teratogenic effects of Curcuma longa extract on zebrafish (Danio rerio)
title_short Phytochemical evaluation, embryotoxicity and teratogenic effects of Curcuma longa extract on zebrafish (Danio rerio)
title_full Phytochemical evaluation, embryotoxicity and teratogenic effects of Curcuma longa extract on zebrafish (Danio rerio)
title_fullStr Phytochemical evaluation, embryotoxicity and teratogenic effects of Curcuma longa extract on zebrafish (Danio rerio)
title_full_unstemmed Phytochemical evaluation, embryotoxicity and teratogenic effects of Curcuma longa extract on zebrafish (Danio rerio)
title_sort phytochemical evaluation, embryotoxicity and teratogenic effects of curcuma longa extract on zebrafish (danio rerio)
publisher Hindawi Limited
publishDate 2019
url http://psasir.upm.edu.my/id/eprint/81739/1/Phytochemical%20evaluation%2C%20embryotoxicity.pdf
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score 13.4562235

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