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Velogenic newcastle disease virus tissue tropism and pathogenesis of infection in chickens by application of in situ PCR, immunoperoxase staining and HE staining

Limited deep studies are available in the field of early stages of pathogenesis of Newcastle disease virus (NDV) infection and tissue tropism of NDV. In this study, 24 specific pathogen free (SPF) chickens of white leghorn breed were infected with Newcastle disease (ND) by intranasal administration...

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Main Authors: Hussein, Elawad A., Bejo, Mohd Hair, Omar, Abdul Rahman, Arshad, Siti Suri, Hani, Homayoun, Balakrishnan, Krishnan Nair, Yakubu, Yusuf, Saeed, Mohammed Ibrahim, Ideris, Aini
Format: Article
Language:English
Published: Elsevier 2019
Online Access:http://psasir.upm.edu.my/id/eprint/82715/1/Velogenic%20newcastle%20disease%20.pdf
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spelling oai:psasir.upm.edu.my:82715 http://psasir.upm.edu.my/id/eprint/82715/ Velogenic newcastle disease virus tissue tropism and pathogenesis of infection in chickens by application of in situ PCR, immunoperoxase staining and HE staining Hussein, Elawad A. Bejo, Mohd Hair Omar, Abdul Rahman Arshad, Siti Suri Hani, Homayoun Balakrishnan, Krishnan Nair Yakubu, Yusuf Saeed, Mohammed Ibrahim Ideris, Aini Limited deep studies are available in the field of early stages of pathogenesis of Newcastle disease virus (NDV) infection and tissue tropism of NDV. In this study, 24 specific pathogen free (SPF) chickens of white leghorn breed were infected with Newcastle disease (ND) by intranasal administration of 10⁵ 50% EID50/0.1 mL of velogenic NDV (vNDV). A second group of 15 chickens were kept as a control group. Chickens were monitored every day to record clinical signs. Infected chickens were euthanized by cervical dislocation at successive times, namely at hours (hrs) 2, 4, 6, 12, days 1, 2, 4, and 6 post-inoculation (pi). Whereas, control group chickens were euthanized on days 0, 1, 2, 4, and 6 pi. Tissues of brain, trachea, lung, caecal tonsil, liver, kidney, spleen, heart, proventriculus, intestine, and thymus were collected, fixed in 10% buffered formalin, embedded in paraffin, and sectioned. HS staining, immunoperoxidase staining (IPS) and in situ PCR were applied. It was concluded that at hr 2 pi, virus seemed to be inclined to trachea and respiratory tract. Meanwhile, it attacked caecal tonsils, intestine and bursa of Fabricus. While primary viraemia was ongoing, virus created footing in kidney and thymus. At hr 4 pi, proventriculus, liver, and spleen were attacked. However, at hr 6 pi, brain and heart were involved. Secondary viraemia probably started as early as hr 12 pi since all collected tissues were positive. Tissue tropism was determined in trachea, caecal tonsil, liver, bursa of Fabricius, intestine, proventriculus, lung, spleen, thymus, kidney, heart, and brain. Elsevier 2019-04 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/82715/1/Velogenic%20newcastle%20disease%20.pdf Hussein, Elawad A. and Bejo, Mohd Hair and Omar, Abdul Rahman and Arshad, Siti Suri and Hani, Homayoun and Balakrishnan, Krishnan Nair and Yakubu, Yusuf and Saeed, Mohammed Ibrahim and Ideris, Aini (2019) Velogenic newcastle disease virus tissue tropism and pathogenesis of infection in chickens by application of in situ PCR, immunoperoxase staining and HE staining. Microbial Pathogenesis, 129. pp. 213-223. ISSN 0882-4010; ESSN 1096-1208 https://www.sciencedirect.com/science/article/abs/pii/S0882401018312555?via%3Dihub 10.1016/j.micpath.2019.02.017
institution UPM IR
collection UPM IR
language English
description Limited deep studies are available in the field of early stages of pathogenesis of Newcastle disease virus (NDV) infection and tissue tropism of NDV. In this study, 24 specific pathogen free (SPF) chickens of white leghorn breed were infected with Newcastle disease (ND) by intranasal administration of 10⁵ 50% EID50/0.1 mL of velogenic NDV (vNDV). A second group of 15 chickens were kept as a control group. Chickens were monitored every day to record clinical signs. Infected chickens were euthanized by cervical dislocation at successive times, namely at hours (hrs) 2, 4, 6, 12, days 1, 2, 4, and 6 post-inoculation (pi). Whereas, control group chickens were euthanized on days 0, 1, 2, 4, and 6 pi. Tissues of brain, trachea, lung, caecal tonsil, liver, kidney, spleen, heart, proventriculus, intestine, and thymus were collected, fixed in 10% buffered formalin, embedded in paraffin, and sectioned. HS staining, immunoperoxidase staining (IPS) and in situ PCR were applied. It was concluded that at hr 2 pi, virus seemed to be inclined to trachea and respiratory tract. Meanwhile, it attacked caecal tonsils, intestine and bursa of Fabricus. While primary viraemia was ongoing, virus created footing in kidney and thymus. At hr 4 pi, proventriculus, liver, and spleen were attacked. However, at hr 6 pi, brain and heart were involved. Secondary viraemia probably started as early as hr 12 pi since all collected tissues were positive. Tissue tropism was determined in trachea, caecal tonsil, liver, bursa of Fabricius, intestine, proventriculus, lung, spleen, thymus, kidney, heart, and brain.
format Article
author Hussein, Elawad A.
Bejo, Mohd Hair
Omar, Abdul Rahman
Arshad, Siti Suri
Hani, Homayoun
Balakrishnan, Krishnan Nair
Yakubu, Yusuf
Saeed, Mohammed Ibrahim
Ideris, Aini
spellingShingle Hussein, Elawad A.
Bejo, Mohd Hair
Omar, Abdul Rahman
Arshad, Siti Suri
Hani, Homayoun
Balakrishnan, Krishnan Nair
Yakubu, Yusuf
Saeed, Mohammed Ibrahim
Ideris, Aini
Velogenic newcastle disease virus tissue tropism and pathogenesis of infection in chickens by application of in situ PCR, immunoperoxase staining and HE staining
author_facet Hussein, Elawad A.
Bejo, Mohd Hair
Omar, Abdul Rahman
Arshad, Siti Suri
Hani, Homayoun
Balakrishnan, Krishnan Nair
Yakubu, Yusuf
Saeed, Mohammed Ibrahim
Ideris, Aini
author_sort Hussein, Elawad A.
title Velogenic newcastle disease virus tissue tropism and pathogenesis of infection in chickens by application of in situ PCR, immunoperoxase staining and HE staining
title_short Velogenic newcastle disease virus tissue tropism and pathogenesis of infection in chickens by application of in situ PCR, immunoperoxase staining and HE staining
title_full Velogenic newcastle disease virus tissue tropism and pathogenesis of infection in chickens by application of in situ PCR, immunoperoxase staining and HE staining
title_fullStr Velogenic newcastle disease virus tissue tropism and pathogenesis of infection in chickens by application of in situ PCR, immunoperoxase staining and HE staining
title_full_unstemmed Velogenic newcastle disease virus tissue tropism and pathogenesis of infection in chickens by application of in situ PCR, immunoperoxase staining and HE staining
title_sort velogenic newcastle disease virus tissue tropism and pathogenesis of infection in chickens by application of in situ pcr, immunoperoxase staining and he staining
publisher Elsevier
publishDate 2019
url http://psasir.upm.edu.my/id/eprint/82715/1/Velogenic%20newcastle%20disease%20.pdf
_version_ 1782759612048474112
score 12.935284

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