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The assessment of cholinesterase from the liver of Anabas testudineus for detection of metal ions

Climbing Perch or its scientific name, Anabas testudineus is classified as one of the freshwater fishes belonging to the family of Anabantidae. It is locally known as „Ikan Puyu‟ and is widely distributed in ponds, swamps and estuaries in Asia. In this study, cholinesterase (ChE) was partially purif...

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Main Author: Wong, Yoong Fei
Format: Project Paper Report
Language:English
Published: 2015
Online Access:http://psasir.upm.edu.my/id/eprint/85098/1/FBSB%202015%2087%20-%20IR.pdf
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spelling oai:psasir.upm.edu.my:85098 http://psasir.upm.edu.my/id/eprint/85098/ The assessment of cholinesterase from the liver of Anabas testudineus for detection of metal ions Wong, Yoong Fei Climbing Perch or its scientific name, Anabas testudineus is classified as one of the freshwater fishes belonging to the family of Anabantidae. It is locally known as „Ikan Puyu‟ and is widely distributed in ponds, swamps and estuaries in Asia. In this study, cholinesterase (ChE) was partially purified from the liver of A. testudineus through ion exchange chromatography. This purification method provided recovery yield of 5.35% with a purification fold of 6.6. The optimum conditions for ChE assay were identified to be 2.5 mM butyrylthiocholine iodide (BTC) with pH 8.0 in 0.1 M Tris-HCl buffer at 40°C. Substrate specificity profile also indicated that ChE favours BTC as substrate because it records the highest catalytic efficiency (Vmax/Km). Metal ion inhibition tests were conducted and mercury (Hg) was found to show the highest inhibition effect (87.30%) whereas lead (Pb) showed the lowest inhibition effect (28.01%). The half maximal inhibitory concentration (IC50) value of mercury for partially purified ChE was 0.071 ppm. Result obtained from protein analysis through Native-PAGE has shown ion exchange chromatography as an effective method in partially purified ChE. All these findings showed that partially purified ChE from the liver of A. testudineus is suitable to be used as a bioindicator to detect the presence of metal ions. 2015-06 Project Paper Report NonPeerReviewed text en http://psasir.upm.edu.my/id/eprint/85098/1/FBSB%202015%2087%20-%20IR.pdf Wong, Yoong Fei (2015) The assessment of cholinesterase from the liver of Anabas testudineus for detection of metal ions. [Project Paper Report]
institution UPM IR
collection UPM IR
language English
description Climbing Perch or its scientific name, Anabas testudineus is classified as one of the freshwater fishes belonging to the family of Anabantidae. It is locally known as „Ikan Puyu‟ and is widely distributed in ponds, swamps and estuaries in Asia. In this study, cholinesterase (ChE) was partially purified from the liver of A. testudineus through ion exchange chromatography. This purification method provided recovery yield of 5.35% with a purification fold of 6.6. The optimum conditions for ChE assay were identified to be 2.5 mM butyrylthiocholine iodide (BTC) with pH 8.0 in 0.1 M Tris-HCl buffer at 40°C. Substrate specificity profile also indicated that ChE favours BTC as substrate because it records the highest catalytic efficiency (Vmax/Km). Metal ion inhibition tests were conducted and mercury (Hg) was found to show the highest inhibition effect (87.30%) whereas lead (Pb) showed the lowest inhibition effect (28.01%). The half maximal inhibitory concentration (IC50) value of mercury for partially purified ChE was 0.071 ppm. Result obtained from protein analysis through Native-PAGE has shown ion exchange chromatography as an effective method in partially purified ChE. All these findings showed that partially purified ChE from the liver of A. testudineus is suitable to be used as a bioindicator to detect the presence of metal ions.
format Project Paper Report
author Wong, Yoong Fei
spellingShingle Wong, Yoong Fei
The assessment of cholinesterase from the liver of Anabas testudineus for detection of metal ions
author_facet Wong, Yoong Fei
author_sort Wong, Yoong Fei
title The assessment of cholinesterase from the liver of Anabas testudineus for detection of metal ions
title_short The assessment of cholinesterase from the liver of Anabas testudineus for detection of metal ions
title_full The assessment of cholinesterase from the liver of Anabas testudineus for detection of metal ions
title_fullStr The assessment of cholinesterase from the liver of Anabas testudineus for detection of metal ions
title_full_unstemmed The assessment of cholinesterase from the liver of Anabas testudineus for detection of metal ions
title_sort assessment of cholinesterase from the liver of anabas testudineus for detection of metal ions
publishDate 2015
url http://psasir.upm.edu.my/id/eprint/85098/1/FBSB%202015%2087%20-%20IR.pdf
_version_ 1819300044541526016
score 13.4562235

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