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Leptospira interrogans and Leptospira kirschneri are the dominant Leptospira species causing human leptospirosis in Central Malaysia

Background: Leptospirosis, commonly known as rat-urine disease, is a global but endemic zoonotic disease in the tropics. Despite the historical report of leptospirosis in Malaysia, the information on human-infecting species is limited. Determining the circulating species is important to understand i...

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Autori principali: Philip, Noraini, Bahtiar Affendy, Norliza, Ramli, Siti Nur Alia, Muhamad Arif, Raja, Pappitha, Nagandran, Elanngovan, Renganathan, Pukunan, Mohd Taib, Niazlin, Masri, Siti Norbaya, Yuhana, Muhamad Yazli, Thian, Leslie Lung Than, Seganathirajah, Mithra, Cyrille, Goarant, Neela, Vasantha Kumari
Natura: Articolo
Lingua:English
Pubblicazione: Public Library of Science 2020
Accesso online:http://psasir.upm.edu.my/id/eprint/86566/1/Leptospira%20interrogans.pdf
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spelling oai:psasir.upm.edu.my:86566 http://psasir.upm.edu.my/id/eprint/86566/ Leptospira interrogans and Leptospira kirschneri are the dominant Leptospira species causing human leptospirosis in Central Malaysia Philip, Noraini Bahtiar Affendy, Norliza Ramli, Siti Nur Alia Muhamad Arif Raja, Pappitha Nagandran, Elanngovan Renganathan, Pukunan Mohd Taib, Niazlin Masri, Siti Norbaya Yuhana, Muhamad Yazli Thian, Leslie Lung Than Seganathirajah, Mithra Cyrille, Goarant Neela, Vasantha Kumari Background: Leptospirosis, commonly known as rat-urine disease, is a global but endemic zoonotic disease in the tropics. Despite the historical report of leptospirosis in Malaysia, the information on human-infecting species is limited. Determining the circulating species is important to understand its epidemiology, thereby to strategize appropriate control measures through public health interventions, diagnostics, therapeutics and vaccine development. Methodology/Principle findings: We investigated the human-infecting Leptospira species in blood and serum samples collected from clinically suspected leptospirosis patients admitted to three tertiary care hospitals in Malaysia. From a total of 165 patients, 92 (56%) were confirmed cases of leptospirosis through Microscopic Agglutination Test (MAT) (n = 43; 47%), Polymerase Chain Reaction (PCR) (n = 63; 68%) or both MAT and PCR (n = 14; 15%). The infecting Leptospira spp., determined by partial 16S rDNA (rrs) gene sequencing revealed two pathogenic species namely Leptospira interrogans (n = 44, 70%) and Leptospira kirschneri (n = 17, 27%) and one intermediate species Leptospira wolffii (n = 2, 3%). Multilocus sequence typing (MLST) identified an isolate of L. interrogans as a novel sequence type (ST 265), suggesting that this human-infecting strain has a unique genetic profile different from similar species isolated from rodents so far. Conclusions/Significance: Leptospira interrogans and Leptospira kirschneri were identified as the dominant Leptospira species causing human leptospirosis in Central Malaysia. The existence of novel clinically important ST 265 (infecting human), that is different from rodent L. interrogans strains cautions reservoir(s) of these Leptospira lineages are yet to be identified. Public Library of Science 2020 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/86566/1/Leptospira%20interrogans.pdf Philip, Noraini and Bahtiar Affendy, Norliza and Ramli, Siti Nur Alia and Muhamad Arif and Raja, Pappitha and Nagandran, Elanngovan and Renganathan, Pukunan and Mohd Taib, Niazlin and Masri, Siti Norbaya and Yuhana, Muhamad Yazli and Thian, Leslie Lung Than and Seganathirajah, Mithra and Cyrille, Goarant and Neela, Vasantha Kumari (2020) Leptospira interrogans and Leptospira kirschneri are the dominant Leptospira species causing human leptospirosis in Central Malaysia. PLoS Neglected Tropical Diseases, 14 (3). pp. 1-14. ISSN 1935-2727; ESSN: 1935-2735 https://journals.plos.org/plosntds/article?id=10.1371/journal.pntd.0008197 10.1371/journal.pntd.0008197
institution UPM IR
collection UPM IR
language English
description Background: Leptospirosis, commonly known as rat-urine disease, is a global but endemic zoonotic disease in the tropics. Despite the historical report of leptospirosis in Malaysia, the information on human-infecting species is limited. Determining the circulating species is important to understand its epidemiology, thereby to strategize appropriate control measures through public health interventions, diagnostics, therapeutics and vaccine development. Methodology/Principle findings: We investigated the human-infecting Leptospira species in blood and serum samples collected from clinically suspected leptospirosis patients admitted to three tertiary care hospitals in Malaysia. From a total of 165 patients, 92 (56%) were confirmed cases of leptospirosis through Microscopic Agglutination Test (MAT) (n = 43; 47%), Polymerase Chain Reaction (PCR) (n = 63; 68%) or both MAT and PCR (n = 14; 15%). The infecting Leptospira spp., determined by partial 16S rDNA (rrs) gene sequencing revealed two pathogenic species namely Leptospira interrogans (n = 44, 70%) and Leptospira kirschneri (n = 17, 27%) and one intermediate species Leptospira wolffii (n = 2, 3%). Multilocus sequence typing (MLST) identified an isolate of L. interrogans as a novel sequence type (ST 265), suggesting that this human-infecting strain has a unique genetic profile different from similar species isolated from rodents so far. Conclusions/Significance: Leptospira interrogans and Leptospira kirschneri were identified as the dominant Leptospira species causing human leptospirosis in Central Malaysia. The existence of novel clinically important ST 265 (infecting human), that is different from rodent L. interrogans strains cautions reservoir(s) of these Leptospira lineages are yet to be identified.
format Article
author Philip, Noraini
Bahtiar Affendy, Norliza
Ramli, Siti Nur Alia
Muhamad Arif
Raja, Pappitha
Nagandran, Elanngovan
Renganathan, Pukunan
Mohd Taib, Niazlin
Masri, Siti Norbaya
Yuhana, Muhamad Yazli
Thian, Leslie Lung Than
Seganathirajah, Mithra
Cyrille, Goarant
Neela, Vasantha Kumari
spellingShingle Philip, Noraini
Bahtiar Affendy, Norliza
Ramli, Siti Nur Alia
Muhamad Arif
Raja, Pappitha
Nagandran, Elanngovan
Renganathan, Pukunan
Mohd Taib, Niazlin
Masri, Siti Norbaya
Yuhana, Muhamad Yazli
Thian, Leslie Lung Than
Seganathirajah, Mithra
Cyrille, Goarant
Neela, Vasantha Kumari
Leptospira interrogans and Leptospira kirschneri are the dominant Leptospira species causing human leptospirosis in Central Malaysia
author_facet Philip, Noraini
Bahtiar Affendy, Norliza
Ramli, Siti Nur Alia
Muhamad Arif
Raja, Pappitha
Nagandran, Elanngovan
Renganathan, Pukunan
Mohd Taib, Niazlin
Masri, Siti Norbaya
Yuhana, Muhamad Yazli
Thian, Leslie Lung Than
Seganathirajah, Mithra
Cyrille, Goarant
Neela, Vasantha Kumari
author_sort Philip, Noraini
title Leptospira interrogans and Leptospira kirschneri are the dominant Leptospira species causing human leptospirosis in Central Malaysia
title_short Leptospira interrogans and Leptospira kirschneri are the dominant Leptospira species causing human leptospirosis in Central Malaysia
title_full Leptospira interrogans and Leptospira kirschneri are the dominant Leptospira species causing human leptospirosis in Central Malaysia
title_fullStr Leptospira interrogans and Leptospira kirschneri are the dominant Leptospira species causing human leptospirosis in Central Malaysia
title_full_unstemmed Leptospira interrogans and Leptospira kirschneri are the dominant Leptospira species causing human leptospirosis in Central Malaysia
title_sort leptospira interrogans and leptospira kirschneri are the dominant leptospira species causing human leptospirosis in central malaysia
publisher Public Library of Science
publishDate 2020
url http://psasir.upm.edu.my/id/eprint/86566/1/Leptospira%20interrogans.pdf
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score 13.4562235

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