প্রচ্ছদ

Molecular detection and genetic diversity of toxoplasma gondii oocysts in cat faeces from Klang Valley, Malaysia, using B1 and REP genes in 2018

The major route for Toxoplasma gondii (T. gondii) infection is through the ingestion of foods contaminated with oocyst from cat faeces. The microscopic detection of T. gondii oocysts in cat faeces is challenging, which contributes to the failure of detecting or differentiating it from other related...

সম্পূর্ণ বিবরণ

সংরক্ষণ করুন:
গ্রন্থ-পঞ্জীর বিবরন
প্রধান লেখক: Wana, Mohammed Nasiru, Mohd Moklas, Mohamad Aris, Watanabe, Malaika, Unyah, Ngah Zasmy, Abdullahi, Sharif Alhassan, Issa Alapid, Ashraf Ahmad, Nordin, Norshariza, Basir, Rusliza, Abd Majid, Roslaini
বিন্যাস: প্রবন্ধ
ভাষা:English
প্রকাশিত: Multidisciplinary Digital Publishing Institute 2020
অনলাইন ব্যবহার করুন:http://psasir.upm.edu.my/id/eprint/86812/1/Molecular%20detection%20and%20genetic%20diversity%20of%20toxoplasma.pdf
ট্যাগগুলো: ট্যাগ যুক্ত করুন
কোনো ট্যাগ নেই, প্রথমজন হিসাবে ট্যাগ করুন!
id oai:psasir.upm.edu.my:86812
record_format eprints
spelling oai:psasir.upm.edu.my:86812 http://psasir.upm.edu.my/id/eprint/86812/ Molecular detection and genetic diversity of toxoplasma gondii oocysts in cat faeces from Klang Valley, Malaysia, using B1 and REP genes in 2018 Wana, Mohammed Nasiru Mohd Moklas, Mohamad Aris Watanabe, Malaika Unyah, Ngah Zasmy Abdullahi, Sharif Alhassan Issa Alapid, Ashraf Ahmad Nordin, Norshariza Basir, Rusliza Abd Majid, Roslaini The major route for Toxoplasma gondii (T. gondii) infection is through the ingestion of foods contaminated with oocyst from cat faeces. The microscopic detection of T. gondii oocysts in cat faeces is challenging, which contributes to the failure of detecting or differentiating it from other related coccidian parasites. This study aims to detect T. gondii oocysts in cat faeces using two multicopy-target PCR assays and to evaluate their genetic diversity. Cat faecal (200) samples were collected from pet cats (PCs; 100) and free-roaming cats (FRCs; 100) within Klang Valley, Malaysia, and screened for coccidian oocysts by microscopy using Sheather’s sucrose floatation. PCR assays were performed on each faecal sample, targeting a B1 gene and a repetitive element (REP) gene to confirm T. gondii oocysts. Additionally, the PCR amplicons from the REP gene were sequenced to further confirm T. gondii-positive samples for phylogenetic analysis. Microscopy detected 7/200 (3.5%) T. gondii-like oocysts, while both the B1 gene and the REP gene detected 17/200 (8.5%) samples positive for T. gondii. All samples that were microscopically positive for T. gondii-like oocysts were also shown to be positive by both B1 and REP genes. The BLAST results sequenced for 16/200 (8.0%) PCR-positive T. gondii samples revealed homology and genetic heterogeneity with T. gondii strains in the GenBank, except for only one positive sample that did not show a result. There was almost perfect agreement (k = 0.145) between the two PCR assays targeting the B1 gene and the REP gene. This is the first report on microscopic, molecular detection and genetic diversity of T. gondii from cat faecal samples in Malaysia. In addition, the sensitivities of either the B1 gene or REP gene multicopy-target PCR assays are suitable for the accurate detection of T. gondii from cat faeces. Multidisciplinary Digital Publishing Institute 2020-07-16 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/86812/1/Molecular%20detection%20and%20genetic%20diversity%20of%20toxoplasma.pdf Wana, Mohammed Nasiru and Mohd Moklas, Mohamad Aris and Watanabe, Malaika and Unyah, Ngah Zasmy and Abdullahi, Sharif Alhassan and Issa Alapid, Ashraf Ahmad and Nordin, Norshariza and Basir, Rusliza and Abd Majid, Roslaini (2020) Molecular detection and genetic diversity of toxoplasma gondii oocysts in cat faeces from Klang Valley, Malaysia, using B1 and REP genes in 2018. Pathogens, 9 (7). art. no. 576. pp. 1-16. ISSN 2076-0817 https://www.mdpi.com/2076-0817/9/7/576 10.3390/pathogens9070576
institution UPM IR
collection UPM IR
language English
description The major route for Toxoplasma gondii (T. gondii) infection is through the ingestion of foods contaminated with oocyst from cat faeces. The microscopic detection of T. gondii oocysts in cat faeces is challenging, which contributes to the failure of detecting or differentiating it from other related coccidian parasites. This study aims to detect T. gondii oocysts in cat faeces using two multicopy-target PCR assays and to evaluate their genetic diversity. Cat faecal (200) samples were collected from pet cats (PCs; 100) and free-roaming cats (FRCs; 100) within Klang Valley, Malaysia, and screened for coccidian oocysts by microscopy using Sheather’s sucrose floatation. PCR assays were performed on each faecal sample, targeting a B1 gene and a repetitive element (REP) gene to confirm T. gondii oocysts. Additionally, the PCR amplicons from the REP gene were sequenced to further confirm T. gondii-positive samples for phylogenetic analysis. Microscopy detected 7/200 (3.5%) T. gondii-like oocysts, while both the B1 gene and the REP gene detected 17/200 (8.5%) samples positive for T. gondii. All samples that were microscopically positive for T. gondii-like oocysts were also shown to be positive by both B1 and REP genes. The BLAST results sequenced for 16/200 (8.0%) PCR-positive T. gondii samples revealed homology and genetic heterogeneity with T. gondii strains in the GenBank, except for only one positive sample that did not show a result. There was almost perfect agreement (k = 0.145) between the two PCR assays targeting the B1 gene and the REP gene. This is the first report on microscopic, molecular detection and genetic diversity of T. gondii from cat faecal samples in Malaysia. In addition, the sensitivities of either the B1 gene or REP gene multicopy-target PCR assays are suitable for the accurate detection of T. gondii from cat faeces.
format Article
author Wana, Mohammed Nasiru
Mohd Moklas, Mohamad Aris
Watanabe, Malaika
Unyah, Ngah Zasmy
Abdullahi, Sharif Alhassan
Issa Alapid, Ashraf Ahmad
Nordin, Norshariza
Basir, Rusliza
Abd Majid, Roslaini
spellingShingle Wana, Mohammed Nasiru
Mohd Moklas, Mohamad Aris
Watanabe, Malaika
Unyah, Ngah Zasmy
Abdullahi, Sharif Alhassan
Issa Alapid, Ashraf Ahmad
Nordin, Norshariza
Basir, Rusliza
Abd Majid, Roslaini
Molecular detection and genetic diversity of toxoplasma gondii oocysts in cat faeces from Klang Valley, Malaysia, using B1 and REP genes in 2018
author_facet Wana, Mohammed Nasiru
Mohd Moklas, Mohamad Aris
Watanabe, Malaika
Unyah, Ngah Zasmy
Abdullahi, Sharif Alhassan
Issa Alapid, Ashraf Ahmad
Nordin, Norshariza
Basir, Rusliza
Abd Majid, Roslaini
author_sort Wana, Mohammed Nasiru
title Molecular detection and genetic diversity of toxoplasma gondii oocysts in cat faeces from Klang Valley, Malaysia, using B1 and REP genes in 2018
title_short Molecular detection and genetic diversity of toxoplasma gondii oocysts in cat faeces from Klang Valley, Malaysia, using B1 and REP genes in 2018
title_full Molecular detection and genetic diversity of toxoplasma gondii oocysts in cat faeces from Klang Valley, Malaysia, using B1 and REP genes in 2018
title_fullStr Molecular detection and genetic diversity of toxoplasma gondii oocysts in cat faeces from Klang Valley, Malaysia, using B1 and REP genes in 2018
title_full_unstemmed Molecular detection and genetic diversity of toxoplasma gondii oocysts in cat faeces from Klang Valley, Malaysia, using B1 and REP genes in 2018
title_sort molecular detection and genetic diversity of toxoplasma gondii oocysts in cat faeces from klang valley, malaysia, using b1 and rep genes in 2018
publisher Multidisciplinary Digital Publishing Institute
publishDate 2020
url http://psasir.upm.edu.my/id/eprint/86812/1/Molecular%20detection%20and%20genetic%20diversity%20of%20toxoplasma.pdf
_version_ 1819300145110450176
score 13.4562235

অনুরূপ উপাদানগুলি