Analysis of Salmonella enterica serovar Enteritidis isolates from chickens and chicken meat products in Malaysia using PFGE, and MLST

Background: Salmonella is a very important foodborne pathogen causing illness in humans. The emergence of drug-resistant strains also constitutes a serious worry to global health and livestock productivity. This study investigated Salmonella isolates from chicken and chicken meat products using the...

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Main Authors: Zakaria, Zunita, Hassan, Latiffah, Sharif, Zawiyah, Ahmad, Norazah, Mohd Ali, Rohaya, Amir Husin, Suraya, Abd Hazis, Nor Hazrin, Mohamed Sohaimi, Norfitriah, Abu Bakar, Shafini, Garba, Bashiru
格式: Article
語言:English
出版: BioMed Central 2020
在線閱讀:http://psasir.upm.edu.my/id/eprint/87863/1/ABSTRACT.pdf
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spelling oai:psasir.upm.edu.my:87863 http://psasir.upm.edu.my/id/eprint/87863/ Analysis of Salmonella enterica serovar Enteritidis isolates from chickens and chicken meat products in Malaysia using PFGE, and MLST Zakaria, Zunita Hassan, Latiffah Sharif, Zawiyah Ahmad, Norazah Mohd Ali, Rohaya Amir Husin, Suraya Abd Hazis, Nor Hazrin Mohamed Sohaimi, Norfitriah Abu Bakar, Shafini Garba, Bashiru Background: Salmonella is a very important foodborne pathogen causing illness in humans. The emergence of drug-resistant strains also constitutes a serious worry to global health and livestock productivity. This study investigated Salmonella isolates from chicken and chicken meat products using the phenotypic antimicrobial screening as well as the molecular characteristics of Salmonella isolates. Upon serotyping of the isolates, the antimicrobial susceptibility profiling using a panel of 9 commonly used antimicrobials was done. Subsequently, the molecular profiles of all the isolates were further determined using Pulsed Field Gel Electrophoresis (PFGE) and the Whole Genome Multi-Locus Sequence Type (wgMLST) analysis in order to obtain the sequence types. Results: The PFGE data was input into FPQuest software, and the dendrogram generated was studied for possible genetic relatedness among the isolates. All the isolates were found to belong to the Salmonella Enteritidis serotype with notable resistance to tetracycline, gentamycin, streptomycin, and sulfadimidine. The S. Enteritidis isolates tested predominantly subtyped into the ST11 and ST1925, which was found to be a single cell variant of ST11. The STs were found to occur in chicken meats, foods, and live chicken cloacal swabs, which may indicate the persistence of the bacteria in multiple foci. Conclusion: The data demonstrate the presence of S. Enteritidis among chickens, indicating its preference and reservoir status for enteric Salmonella pathogens. BioMed Central 2020 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/87863/1/ABSTRACT.pdf Zakaria, Zunita and Hassan, Latiffah and Sharif, Zawiyah and Ahmad, Norazah and Mohd Ali, Rohaya and Amir Husin, Suraya and Abd Hazis, Nor Hazrin and Mohamed Sohaimi, Norfitriah and Abu Bakar, Shafini and Garba, Bashiru (2020) Analysis of Salmonella enterica serovar Enteritidis isolates from chickens and chicken meat products in Malaysia using PFGE, and MLST. BMC Veterinary Research, 16. art. no. 393. pp. 1-8. ISSN 1746-6148 https://bmcvetres.biomedcentral.com/articles/10.1186/s12917-020-02605-y 10.1186/s12917-020-02605-y
institution UPM IR
collection UPM IR
language English
description Background: Salmonella is a very important foodborne pathogen causing illness in humans. The emergence of drug-resistant strains also constitutes a serious worry to global health and livestock productivity. This study investigated Salmonella isolates from chicken and chicken meat products using the phenotypic antimicrobial screening as well as the molecular characteristics of Salmonella isolates. Upon serotyping of the isolates, the antimicrobial susceptibility profiling using a panel of 9 commonly used antimicrobials was done. Subsequently, the molecular profiles of all the isolates were further determined using Pulsed Field Gel Electrophoresis (PFGE) and the Whole Genome Multi-Locus Sequence Type (wgMLST) analysis in order to obtain the sequence types. Results: The PFGE data was input into FPQuest software, and the dendrogram generated was studied for possible genetic relatedness among the isolates. All the isolates were found to belong to the Salmonella Enteritidis serotype with notable resistance to tetracycline, gentamycin, streptomycin, and sulfadimidine. The S. Enteritidis isolates tested predominantly subtyped into the ST11 and ST1925, which was found to be a single cell variant of ST11. The STs were found to occur in chicken meats, foods, and live chicken cloacal swabs, which may indicate the persistence of the bacteria in multiple foci. Conclusion: The data demonstrate the presence of S. Enteritidis among chickens, indicating its preference and reservoir status for enteric Salmonella pathogens.
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author Zakaria, Zunita
Hassan, Latiffah
Sharif, Zawiyah
Ahmad, Norazah
Mohd Ali, Rohaya
Amir Husin, Suraya
Abd Hazis, Nor Hazrin
Mohamed Sohaimi, Norfitriah
Abu Bakar, Shafini
Garba, Bashiru
spellingShingle Zakaria, Zunita
Hassan, Latiffah
Sharif, Zawiyah
Ahmad, Norazah
Mohd Ali, Rohaya
Amir Husin, Suraya
Abd Hazis, Nor Hazrin
Mohamed Sohaimi, Norfitriah
Abu Bakar, Shafini
Garba, Bashiru
Analysis of Salmonella enterica serovar Enteritidis isolates from chickens and chicken meat products in Malaysia using PFGE, and MLST
author_facet Zakaria, Zunita
Hassan, Latiffah
Sharif, Zawiyah
Ahmad, Norazah
Mohd Ali, Rohaya
Amir Husin, Suraya
Abd Hazis, Nor Hazrin
Mohamed Sohaimi, Norfitriah
Abu Bakar, Shafini
Garba, Bashiru
author_sort Zakaria, Zunita
title Analysis of Salmonella enterica serovar Enteritidis isolates from chickens and chicken meat products in Malaysia using PFGE, and MLST
title_short Analysis of Salmonella enterica serovar Enteritidis isolates from chickens and chicken meat products in Malaysia using PFGE, and MLST
title_full Analysis of Salmonella enterica serovar Enteritidis isolates from chickens and chicken meat products in Malaysia using PFGE, and MLST
title_fullStr Analysis of Salmonella enterica serovar Enteritidis isolates from chickens and chicken meat products in Malaysia using PFGE, and MLST
title_full_unstemmed Analysis of Salmonella enterica serovar Enteritidis isolates from chickens and chicken meat products in Malaysia using PFGE, and MLST
title_sort analysis of salmonella enterica serovar enteritidis isolates from chickens and chicken meat products in malaysia using pfge, and mlst
publisher BioMed Central
publishDate 2020
url http://psasir.upm.edu.my/id/eprint/87863/1/ABSTRACT.pdf
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