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Combine drug delivery of Thymoquinone-doxorubicin by cockles shell derived pH-sensitive Aragonite CaCO3 nanoparticles

Background: Cockleshell-derived aragonite calcium carbonate nanoparticles were prepared by the top-down approach for combine delivery of two types of drugs. Objective: The aim of this study was to synthesize and characterize thymoquinone-doxorubicin loaded cockle shell-derived aragonite calcium carb...

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Hlavní autoři: Ibiyeye, Kehinde M., Abu Bakar, Md Zuki, Nurdin, Norshariza, Ajat, Mokrish
Médium: Článek
Jazyk:English
Vydáno: Bentham Science 2020
On-line přístup:http://psasir.upm.edu.my/id/eprint/88190/1/ABSTRACT.pdf
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spelling oai:psasir.upm.edu.my:88190 http://psasir.upm.edu.my/id/eprint/88190/ Combine drug delivery of Thymoquinone-doxorubicin by cockles shell derived pH-sensitive Aragonite CaCO3 nanoparticles Ibiyeye, Kehinde M. Abu Bakar, Md Zuki Nurdin, Norshariza Ajat, Mokrish Background: Cockleshell-derived aragonite calcium carbonate nanoparticles were prepared by the top-down approach for combine delivery of two types of drugs. Objective: The aim of this study was to synthesize and characterize thymoquinone-doxorubicin loaded cockle shell-derived aragonite calcium carbonate nanoparticle. Aragonite calcium carbonate nanoparticles encapsulating thymoquinone and doxorubicin alone were also prepared. Methods: The blank and drug-loaded nanoparticles were characterized by field emission scanning electron microscopy, transmission electron microscopy, Zeta potential, Fourier transformed infrared and X-ray diffraction. Drug delivery properties, in vitro drug release study at pH 7.4, 6 and 4.8, and effect of blank nanoparticles on MCF10A, 3T3, MDA MB231 cells were also analyzed. Results: The blank and drug-loaded nanoparticles were pleomorphic and their sizes varying from 53.65 ± 10.29 nm to 60.49 ± 11.36 nm with an overall negative charge. The entrapment efficiency of thymoquinone and doxorubicin were 41.6 and 95.8, respectively. The FTIR showed little alteration after loading thymoquinone and doxorubicin while XRD patterns revealed no changes in the crystallizations of nanoparticles after drug loading. The drug release kinetics of doxorubicin and thymoquinone from the nanoparticles showed a continuous and gradual release after an initial burst release was observed. At pH 4.8, about 100% of drug release was noticed, 70% at pH 6 while only 50% at pH 7.4. The cell viability was 80% at a concentration of 1000 ug/ml of blank nanoparticle. Conclusion: The cockle shell-derived pH sensitive aragonite calcium carbonate nanoparticle provides an effective and simple means of multiple drug delivery and function as a platform for pH controlled release of loaded therapeutic agents. Bentham Science 2020 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/88190/1/ABSTRACT.pdf Ibiyeye, Kehinde M. and Abu Bakar, Md Zuki and Nurdin, Norshariza and Ajat, Mokrish (2020) Combine drug delivery of Thymoquinone-doxorubicin by cockles shell derived pH-sensitive Aragonite CaCO3 nanoparticles. Nanoscience and Nanotechnology - Asia, 10 (4). 518 - 533. ISSN 2210-6812; ESSN: 2210-6820 https://www.eurekaselect.com/article/98414 10.2174/2210681209666190508122540
institution UPM IR
collection UPM IR
language English
description Background: Cockleshell-derived aragonite calcium carbonate nanoparticles were prepared by the top-down approach for combine delivery of two types of drugs. Objective: The aim of this study was to synthesize and characterize thymoquinone-doxorubicin loaded cockle shell-derived aragonite calcium carbonate nanoparticle. Aragonite calcium carbonate nanoparticles encapsulating thymoquinone and doxorubicin alone were also prepared. Methods: The blank and drug-loaded nanoparticles were characterized by field emission scanning electron microscopy, transmission electron microscopy, Zeta potential, Fourier transformed infrared and X-ray diffraction. Drug delivery properties, in vitro drug release study at pH 7.4, 6 and 4.8, and effect of blank nanoparticles on MCF10A, 3T3, MDA MB231 cells were also analyzed. Results: The blank and drug-loaded nanoparticles were pleomorphic and their sizes varying from 53.65 ± 10.29 nm to 60.49 ± 11.36 nm with an overall negative charge. The entrapment efficiency of thymoquinone and doxorubicin were 41.6 and 95.8, respectively. The FTIR showed little alteration after loading thymoquinone and doxorubicin while XRD patterns revealed no changes in the crystallizations of nanoparticles after drug loading. The drug release kinetics of doxorubicin and thymoquinone from the nanoparticles showed a continuous and gradual release after an initial burst release was observed. At pH 4.8, about 100% of drug release was noticed, 70% at pH 6 while only 50% at pH 7.4. The cell viability was 80% at a concentration of 1000 ug/ml of blank nanoparticle. Conclusion: The cockle shell-derived pH sensitive aragonite calcium carbonate nanoparticle provides an effective and simple means of multiple drug delivery and function as a platform for pH controlled release of loaded therapeutic agents.
format Article
author Ibiyeye, Kehinde M.
Abu Bakar, Md Zuki
Nurdin, Norshariza
Ajat, Mokrish
spellingShingle Ibiyeye, Kehinde M.
Abu Bakar, Md Zuki
Nurdin, Norshariza
Ajat, Mokrish
Combine drug delivery of Thymoquinone-doxorubicin by cockles shell derived pH-sensitive Aragonite CaCO3 nanoparticles
author_facet Ibiyeye, Kehinde M.
Abu Bakar, Md Zuki
Nurdin, Norshariza
Ajat, Mokrish
author_sort Ibiyeye, Kehinde M.
title Combine drug delivery of Thymoquinone-doxorubicin by cockles shell derived pH-sensitive Aragonite CaCO3 nanoparticles
title_short Combine drug delivery of Thymoquinone-doxorubicin by cockles shell derived pH-sensitive Aragonite CaCO3 nanoparticles
title_full Combine drug delivery of Thymoquinone-doxorubicin by cockles shell derived pH-sensitive Aragonite CaCO3 nanoparticles
title_fullStr Combine drug delivery of Thymoquinone-doxorubicin by cockles shell derived pH-sensitive Aragonite CaCO3 nanoparticles
title_full_unstemmed Combine drug delivery of Thymoquinone-doxorubicin by cockles shell derived pH-sensitive Aragonite CaCO3 nanoparticles
title_sort combine drug delivery of thymoquinone-doxorubicin by cockles shell derived ph-sensitive aragonite caco3 nanoparticles
publisher Bentham Science
publishDate 2020
url http://psasir.upm.edu.my/id/eprint/88190/1/ABSTRACT.pdf
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score 13.4562235

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