Cover Image

Electrotransformation on selected plant growth-promoting Rhizobacteria (PGPR) and Chromobacterium violaceum

Electrotransformation was successfully transferred a 21.6 kb cosmid vector pLAFR1 into the selected Plant Growth-Promoting Rhizobacteria (PGPR) and Chromobacterium violaceum with maximum efficiency about 107 transformants/µg at electric field strength of 10kV/cm for shorter pulse length range betwee...

Full description

Saved in:
Bibliographic Details
Main Authors: Loke, Wai Keong, Mohd Saud, Halimi
Format: Article
Language:English
Published: Penerbit Universiti Sultan Zainal Abidin 2020
Online Access:http://psasir.upm.edu.my/id/eprint/88779/1/ABSTRACT.pdf
Tags: Add Tag
No Tags, Be the first to tag this record!
id oai:psasir.upm.edu.my:88779
record_format eprints
spelling oai:psasir.upm.edu.my:88779 http://psasir.upm.edu.my/id/eprint/88779/ Electrotransformation on selected plant growth-promoting Rhizobacteria (PGPR) and Chromobacterium violaceum Loke, Wai Keong Mohd Saud, Halimi Electrotransformation was successfully transferred a 21.6 kb cosmid vector pLAFR1 into the selected Plant Growth-Promoting Rhizobacteria (PGPR) and Chromobacterium violaceum with maximum efficiency about 107 transformants/µg at electric field strength of 10kV/cm for shorter pulse length range between 4.1 to 4.4 milliseconds and about 108 transformants/µg at electric field strength of 5kV/cm for a longer pulse length range between 8.5 to 8.7 milliseconds. Longer incubation period will increase the transformation efficiency but continuing increasing the period after 20 hours will decrease the transformation efficiency. Penerbit Universiti Sultan Zainal Abidin 2020 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/88779/1/ABSTRACT.pdf Loke, Wai Keong and Mohd Saud, Halimi (2020) Electrotransformation on selected plant growth-promoting Rhizobacteria (PGPR) and Chromobacterium violaceum. Journal of Agrobiotechnology, 11 (1). 63 - 69. ISSN 2180-1983 https://journal.unisza.edu.my/agrobiotechnology/index.php/agrobiotechnology/article/view/214 10.37231/jab.2020.11.1.214
institution UPM IR
collection UPM IR
language English
description Electrotransformation was successfully transferred a 21.6 kb cosmid vector pLAFR1 into the selected Plant Growth-Promoting Rhizobacteria (PGPR) and Chromobacterium violaceum with maximum efficiency about 107 transformants/µg at electric field strength of 10kV/cm for shorter pulse length range between 4.1 to 4.4 milliseconds and about 108 transformants/µg at electric field strength of 5kV/cm for a longer pulse length range between 8.5 to 8.7 milliseconds. Longer incubation period will increase the transformation efficiency but continuing increasing the period after 20 hours will decrease the transformation efficiency.
format Article
author Loke, Wai Keong
Mohd Saud, Halimi
spellingShingle Loke, Wai Keong
Mohd Saud, Halimi
Electrotransformation on selected plant growth-promoting Rhizobacteria (PGPR) and Chromobacterium violaceum
author_facet Loke, Wai Keong
Mohd Saud, Halimi
author_sort Loke, Wai Keong
title Electrotransformation on selected plant growth-promoting Rhizobacteria (PGPR) and Chromobacterium violaceum
title_short Electrotransformation on selected plant growth-promoting Rhizobacteria (PGPR) and Chromobacterium violaceum
title_full Electrotransformation on selected plant growth-promoting Rhizobacteria (PGPR) and Chromobacterium violaceum
title_fullStr Electrotransformation on selected plant growth-promoting Rhizobacteria (PGPR) and Chromobacterium violaceum
title_full_unstemmed Electrotransformation on selected plant growth-promoting Rhizobacteria (PGPR) and Chromobacterium violaceum
title_sort electrotransformation on selected plant growth-promoting rhizobacteria (pgpr) and chromobacterium violaceum
publisher Penerbit Universiti Sultan Zainal Abidin
publishDate 2020
url http://psasir.upm.edu.my/id/eprint/88779/1/ABSTRACT.pdf
_version_ 1819300282271531008
score 13.4562235

Similar Items