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Evaluation of anti-parasitic effect of Andrographis paniculata (Burm. F) nees, ethanolic extract of toxoplasma gondii

Infection with Toxoplasma gondii remains widespread because water and food serve as major sources of sporulated oocysts. Infection is poorly controlled as current medications have limited efficacious and severe side effects, and are accompanied by potential development of resistance, and there is an...

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Auteurs principaux: Abdullahi, Sharif Alhassan, Unyah, Ngah Zasmy, Nordin, Norshariza, Mohammed Nasiru, Wana, Alapid, Ashraf, Basir, Rusliza, Abd Majid, Roslaini
Format: Article
Langue:English
Publié: Southeast Asian Ministers of Education Organisation 2020
Accès en ligne:http://psasir.upm.edu.my/id/eprint/88864/1/ABSTRACT.pdf
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spelling oai:psasir.upm.edu.my:88864 http://psasir.upm.edu.my/id/eprint/88864/ Evaluation of anti-parasitic effect of Andrographis paniculata (Burm. F) nees, ethanolic extract of toxoplasma gondii Abdullahi, Sharif Alhassan Unyah, Ngah Zasmy Nordin, Norshariza Mohammed Nasiru, Wana Alapid, Ashraf Basir, Rusliza Abd Majid, Roslaini Infection with Toxoplasma gondii remains widespread because water and food serve as major sources of sporulated oocysts. Infection is poorly controlled as current medications have limited efficacious and severe side effects, and are accompanied by potential development of resistance, and there is an absence of vaccine. The effects of ethanolic extract of Andrographis paniculata (EEAP) on T. gondii in vitro were evaluated. EEAP, obtained through maceration of leaf powder with 100% ethanol for three days, contained an alkaloid, phytosterols and phytophenolic compounds. IC50 (50% inhibitory concentration) of EEAP against Vero cells was 142 µg/ml and against T. gondii RH strain growth in Vero cells was 4.4 µg/ml (selectivity index = 35) compared to 8.3 µg/ml for clindamycin. EEAP (4.4 µg/ml) inhibited tachyzoite cell invasion and intracellular proliferation >70 and 80% respectively compared to control following a 48-hour treatment. These findings showed that EEAP contains promising drug candidates effective against T. gondii and was safe to host cells. Southeast Asian Ministers of Education Organisation 2020 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/88864/1/ABSTRACT.pdf Abdullahi, Sharif Alhassan and Unyah, Ngah Zasmy and Nordin, Norshariza and Mohammed Nasiru, Wana and Alapid, Ashraf and Basir, Rusliza and Abd Majid, Roslaini (2020) Evaluation of anti-parasitic effect of Andrographis paniculata (Burm. F) nees, ethanolic extract of toxoplasma gondii. Southeast Asian Journal of Tropical Medicine and Public Health, 51 (5). 675 - 685. ISSN 0125-1562 https://journal.seameotropmednetwork.org/index.php/jtropmed/article/view/255
institution UPM IR
collection UPM IR
language English
description Infection with Toxoplasma gondii remains widespread because water and food serve as major sources of sporulated oocysts. Infection is poorly controlled as current medications have limited efficacious and severe side effects, and are accompanied by potential development of resistance, and there is an absence of vaccine. The effects of ethanolic extract of Andrographis paniculata (EEAP) on T. gondii in vitro were evaluated. EEAP, obtained through maceration of leaf powder with 100% ethanol for three days, contained an alkaloid, phytosterols and phytophenolic compounds. IC50 (50% inhibitory concentration) of EEAP against Vero cells was 142 µg/ml and against T. gondii RH strain growth in Vero cells was 4.4 µg/ml (selectivity index = 35) compared to 8.3 µg/ml for clindamycin. EEAP (4.4 µg/ml) inhibited tachyzoite cell invasion and intracellular proliferation >70 and 80% respectively compared to control following a 48-hour treatment. These findings showed that EEAP contains promising drug candidates effective against T. gondii and was safe to host cells.
format Article
author Abdullahi, Sharif Alhassan
Unyah, Ngah Zasmy
Nordin, Norshariza
Mohammed Nasiru, Wana
Alapid, Ashraf
Basir, Rusliza
Abd Majid, Roslaini
spellingShingle Abdullahi, Sharif Alhassan
Unyah, Ngah Zasmy
Nordin, Norshariza
Mohammed Nasiru, Wana
Alapid, Ashraf
Basir, Rusliza
Abd Majid, Roslaini
Evaluation of anti-parasitic effect of Andrographis paniculata (Burm. F) nees, ethanolic extract of toxoplasma gondii
author_facet Abdullahi, Sharif Alhassan
Unyah, Ngah Zasmy
Nordin, Norshariza
Mohammed Nasiru, Wana
Alapid, Ashraf
Basir, Rusliza
Abd Majid, Roslaini
author_sort Abdullahi, Sharif Alhassan
title Evaluation of anti-parasitic effect of Andrographis paniculata (Burm. F) nees, ethanolic extract of toxoplasma gondii
title_short Evaluation of anti-parasitic effect of Andrographis paniculata (Burm. F) nees, ethanolic extract of toxoplasma gondii
title_full Evaluation of anti-parasitic effect of Andrographis paniculata (Burm. F) nees, ethanolic extract of toxoplasma gondii
title_fullStr Evaluation of anti-parasitic effect of Andrographis paniculata (Burm. F) nees, ethanolic extract of toxoplasma gondii
title_full_unstemmed Evaluation of anti-parasitic effect of Andrographis paniculata (Burm. F) nees, ethanolic extract of toxoplasma gondii
title_sort evaluation of anti-parasitic effect of andrographis paniculata (burm. f) nees, ethanolic extract of toxoplasma gondii
publisher Southeast Asian Ministers of Education Organisation
publishDate 2020
url http://psasir.upm.edu.my/id/eprint/88864/1/ABSTRACT.pdf
_version_ 1819300288749633536
score 13.4562235

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