Personeel: First report of pathogenic Leptospira spp. isolated from urine and kidneys of naturally infected cats

First report of pathogenic Leptospira spp. isolated from urine and kidneys of naturally infected cats

Leptospirosis is one of the most widespread zoonotic diseases and can infect both humans and animals worldwide. Healthy cat, as a potential source of exposure to humans, are likely underestimated owing to the lack of overt clinical signs associated with Leptospira spp. infection in this species. The...

Volledige beschrijving

Bewaard in:

Bibliografische gegevens
Hoofdauteurs:	Alashraf, Abdul Rahman, Lau, Seng Fong, Bejo, Siti Khairani, Khor, Kuan Hua, Ajat, Mokrish, Radzi, Rozanaliza, Roslan, Muhammad Azri, Abdul Rahman, Muhammad Sabri
Formaat:	Artikel
Taal:	English
Gepubliceerd in:	Public Library of Science 2020
Online toegang:	http://psasir.upm.edu.my/id/eprint/89032/1/LEPTOS.pdf
Tags:	Voeg label toe Geen labels, Wees de eerste die dit record labelt!

id	oai:psasir.upm.edu.my:89032
record_format	eprints
spelling	oai:psasir.upm.edu.my:89032 http://psasir.upm.edu.my/id/eprint/89032/ First report of pathogenic Leptospira spp. isolated from urine and kidneys of naturally infected cats Alashraf, Abdul Rahman Lau, Seng Fong Bejo, Siti Khairani Khor, Kuan Hua Ajat, Mokrish Radzi, Rozanaliza Roslan, Muhammad Azri Abdul Rahman, Muhammad Sabri Leptospirosis is one of the most widespread zoonotic diseases and can infect both humans and animals worldwide. Healthy cat, as a potential source of exposure to humans, are likely underestimated owing to the lack of overt clinical signs associated with Leptospira spp. infection in this species. The aim of the study was to determine the exposure, shedding, and carrier status of leptospires in shelter cats in Malaysia by using serological, molecular, and bacteriological methods. For this study, 82 healthy cats from two shelters were sampled. The blood, urine, and kidneys were tested using the microscopic agglutination test (MAT), polymerase chain reaction (PCR), and bacterial culture. On the basis of serological, molecular, and/or culture techniques, the total detection of leptospiral infection was 29.3% (n = 24/82). Through culture techniques, 16.7% (n = 4/24) of the cats that tested positive were carriers with positive kidney cultures, and one cat was culture positive for both urine and kidney. The Leptospira spp. isolates were identified as pathogenic L. interrogans serovar Bataviae through serological and molecular methods. Through serological techniques, 87.5% (n = 21/24) had positive antibody titers (100–1600) and most of the Bataviae serogroup (n = 19/21). Using PCR, 16.7% (n = 4/24) of cats were shown to have pathogenic Leptospira spp. DNA in their urine. Furthermore, three out of four culture positive cats were serology negative. The present study reports the first retrieval of pathogenic leptospires from urine and kidneys obtained from naturally infected cats. The results provide evidence of the potential role of naturally infected cats in the transmission of leptospires. Additionally, leptospiral infection occurs sub-clinically in cats. The culture isolation provides evidence that healthy cats could be reservoirs of leptospiral infection, and this information may promote the development of disease prevention strategies for the cat population. Public Library of Science 2020 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/89032/1/LEPTOS.pdf Alashraf, Abdul Rahman and Lau, Seng Fong and Bejo, Siti Khairani and Khor, Kuan Hua and Ajat, Mokrish and Radzi, Rozanaliza and Roslan, Muhammad Azri and Abdul Rahman, Muhammad Sabri (2020) First report of pathogenic Leptospira spp. isolated from urine and kidneys of naturally infected cats. PLoS One, 15 (3). pp. 1-13. ISSN 1932-6203 https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0230048 https://doi.org/10.1371/journal.pone.0230048
institution	UPM IR
collection	UPM IR
language	English
description	Leptospirosis is one of the most widespread zoonotic diseases and can infect both humans and animals worldwide. Healthy cat, as a potential source of exposure to humans, are likely underestimated owing to the lack of overt clinical signs associated with Leptospira spp. infection in this species. The aim of the study was to determine the exposure, shedding, and carrier status of leptospires in shelter cats in Malaysia by using serological, molecular, and bacteriological methods. For this study, 82 healthy cats from two shelters were sampled. The blood, urine, and kidneys were tested using the microscopic agglutination test (MAT), polymerase chain reaction (PCR), and bacterial culture. On the basis of serological, molecular, and/or culture techniques, the total detection of leptospiral infection was 29.3% (n = 24/82). Through culture techniques, 16.7% (n = 4/24) of the cats that tested positive were carriers with positive kidney cultures, and one cat was culture positive for both urine and kidney. The Leptospira spp. isolates were identified as pathogenic L. interrogans serovar Bataviae through serological and molecular methods. Through serological techniques, 87.5% (n = 21/24) had positive antibody titers (100–1600) and most of the Bataviae serogroup (n = 19/21). Using PCR, 16.7% (n = 4/24) of cats were shown to have pathogenic Leptospira spp. DNA in their urine. Furthermore, three out of four culture positive cats were serology negative. The present study reports the first retrieval of pathogenic leptospires from urine and kidneys obtained from naturally infected cats. The results provide evidence of the potential role of naturally infected cats in the transmission of leptospires. Additionally, leptospiral infection occurs sub-clinically in cats. The culture isolation provides evidence that healthy cats could be reservoirs of leptospiral infection, and this information may promote the development of disease prevention strategies for the cat population.
format	Article
author	Alashraf, Abdul Rahman Lau, Seng Fong Bejo, Siti Khairani Khor, Kuan Hua Ajat, Mokrish Radzi, Rozanaliza Roslan, Muhammad Azri Abdul Rahman, Muhammad Sabri
spellingShingle	Alashraf, Abdul Rahman Lau, Seng Fong Bejo, Siti Khairani Khor, Kuan Hua Ajat, Mokrish Radzi, Rozanaliza Roslan, Muhammad Azri Abdul Rahman, Muhammad Sabri First report of pathogenic Leptospira spp. isolated from urine and kidneys of naturally infected cats
author_facet	Alashraf, Abdul Rahman Lau, Seng Fong Bejo, Siti Khairani Khor, Kuan Hua Ajat, Mokrish Radzi, Rozanaliza Roslan, Muhammad Azri Abdul Rahman, Muhammad Sabri
author_sort	Alashraf, Abdul Rahman
title	First report of pathogenic Leptospira spp. isolated from urine and kidneys of naturally infected cats
title_short	First report of pathogenic Leptospira spp. isolated from urine and kidneys of naturally infected cats
title_full	First report of pathogenic Leptospira spp. isolated from urine and kidneys of naturally infected cats
title_fullStr	First report of pathogenic Leptospira spp. isolated from urine and kidneys of naturally infected cats
title_full_unstemmed	First report of pathogenic Leptospira spp. isolated from urine and kidneys of naturally infected cats
title_sort	first report of pathogenic leptospira spp. isolated from urine and kidneys of naturally infected cats
publisher	Public Library of Science
publishDate	2020
url	http://psasir.upm.edu.my/id/eprint/89032/1/LEPTOS.pdf
_version_	1819300302817329152
score	13.4562235

First report of pathogenic Leptospira spp. isolated from urine and kidneys of naturally infected cats

Gelijkaardige items