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Determination of the genomic structure of homogentisate geranylgeranyl transferase gene of oil palm

Elaeis guineensis originated from West Africa is the only species that was planted in the Malaysian plantation as a commercial oil palm. Since 1971, Malaysia has been the largest producer and exporter of oil palm. Oil palm is rich in vitamin E that consists of two major molecules which are tocophero...

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Autor principal: Abdullah, Mohd Fauze Hazis
Formato: Project Paper Report
Idioma:English
Publicado em: 2015
Acesso em linha:http://psasir.upm.edu.my/id/eprint/91004/1/FP%202015%20145%20-%20IR.pdf
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spelling oai:psasir.upm.edu.my:91004 http://psasir.upm.edu.my/id/eprint/91004/ Determination of the genomic structure of homogentisate geranylgeranyl transferase gene of oil palm Abdullah, Mohd Fauze Hazis Elaeis guineensis originated from West Africa is the only species that was planted in the Malaysian plantation as a commercial oil palm. Since 1971, Malaysia has been the largest producer and exporter of oil palm. Oil palm is rich in vitamin E that consists of two major molecules which are tocopherols and tocotrienols. Oil palm is the richest source of tocotrienol. Homogentisate geranylgeranyl transferase (HGGT) is the enzyme that initiates tocotrienol synthesis in monocot seeds such as oil palm. The main objective of this study was to compare the genomic structure of HGGT gene of oil palm with other genomic structure of HGGT obtained from the previous study by using polymerase chain reaction (PCR) based method. Young leaves of oil palm were collected from the plantation area at Universiti Putra Malaysia, Serdang, Selangor, Malaysia and the leaves were extracted by using modified cetyltrimethyl ammonium bromide (CTAB). From this study, the genomic structure obtained was not exactly the same with the previous study due to the sequencing errors or the presence of introns in the sequence. From the alignment between genomic and the cDNA sequence, the introns that separate the coding sequence was able to be determined. 2015 Project Paper Report NonPeerReviewed text en http://psasir.upm.edu.my/id/eprint/91004/1/FP%202015%20145%20-%20IR.pdf Abdullah, Mohd Fauze Hazis (2015) Determination of the genomic structure of homogentisate geranylgeranyl transferase gene of oil palm. [Project Paper Report]
institution UPM IR
collection UPM IR
language English
description Elaeis guineensis originated from West Africa is the only species that was planted in the Malaysian plantation as a commercial oil palm. Since 1971, Malaysia has been the largest producer and exporter of oil palm. Oil palm is rich in vitamin E that consists of two major molecules which are tocopherols and tocotrienols. Oil palm is the richest source of tocotrienol. Homogentisate geranylgeranyl transferase (HGGT) is the enzyme that initiates tocotrienol synthesis in monocot seeds such as oil palm. The main objective of this study was to compare the genomic structure of HGGT gene of oil palm with other genomic structure of HGGT obtained from the previous study by using polymerase chain reaction (PCR) based method. Young leaves of oil palm were collected from the plantation area at Universiti Putra Malaysia, Serdang, Selangor, Malaysia and the leaves were extracted by using modified cetyltrimethyl ammonium bromide (CTAB). From this study, the genomic structure obtained was not exactly the same with the previous study due to the sequencing errors or the presence of introns in the sequence. From the alignment between genomic and the cDNA sequence, the introns that separate the coding sequence was able to be determined.
format Project Paper Report
author Abdullah, Mohd Fauze Hazis
spellingShingle Abdullah, Mohd Fauze Hazis
Determination of the genomic structure of homogentisate geranylgeranyl transferase gene of oil palm
author_facet Abdullah, Mohd Fauze Hazis
author_sort Abdullah, Mohd Fauze Hazis
title Determination of the genomic structure of homogentisate geranylgeranyl transferase gene of oil palm
title_short Determination of the genomic structure of homogentisate geranylgeranyl transferase gene of oil palm
title_full Determination of the genomic structure of homogentisate geranylgeranyl transferase gene of oil palm
title_fullStr Determination of the genomic structure of homogentisate geranylgeranyl transferase gene of oil palm
title_full_unstemmed Determination of the genomic structure of homogentisate geranylgeranyl transferase gene of oil palm
title_sort determination of the genomic structure of homogentisate geranylgeranyl transferase gene of oil palm
publishDate 2015
url http://psasir.upm.edu.my/id/eprint/91004/1/FP%202015%20145%20-%20IR.pdf
_version_ 1819300467275988992
score 13.4562235

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