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Antibacterial activity of Parkia speciosa Hassk pod crude extract against Pseudomonas fuscovaginae

Rice is one of the most important food crops that feed almost over 3 billion of people worldwide. However, the outbreak of pest and diseases affected the world rice production. One of the serious infection on paddy plant is Bacterial Sheath Brown Rot caused by Pseudomonas fuscovaginae. Pseudomonas f...

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Main Author: Kah, Oi Yuan
Format: Project Paper Report
Language:English
Published: 2016
Online Access:http://psasir.upm.edu.my/id/eprint/91543/1/FP%202016%2026%20-%20IR.pdf
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spelling oai:psasir.upm.edu.my:91543 http://psasir.upm.edu.my/id/eprint/91543/ Antibacterial activity of Parkia speciosa Hassk pod crude extract against Pseudomonas fuscovaginae Kah, Oi Yuan Rice is one of the most important food crops that feed almost over 3 billion of people worldwide. However, the outbreak of pest and diseases affected the world rice production. One of the serious infection on paddy plant is Bacterial Sheath Brown Rot caused by Pseudomonas fuscovaginae. Pseudomonas fuscovaginae is a florescent gram negative pathogenic bacterium. Several control methods had been carried out and found to be effective toward Pseudomonas fuscovaginae yet does not completely eliminate the pathogen from the plant. Parkia speciosa Hassk has been used as folk medicine to treat various human diseases and sickness. Alkaloid, Saponin, Terpenoids, Phenolic, Flavonoid, and Tannin were the phytochemical substances that can be found in all parts of Parkia speciosa plant. Several research had found that methanolic extract of Parkia speciosa pod can inhibit the growth of bacteria Staphylococcus aureus and Escherichia coli. However, there is no research on antibacterial activities of this plant extract toward plant pathogenic bacteria. This study is to identify the antibacterial activities of Parkia speciosa pod extract toward Pseudomonas fuscovaginae and to determine the active chemical compounds in the Parkia speciosa pod. Methanolic pod extract of Parkia speciosa with concentration of 25, 50, 100, 150, 200,250, 300, 350, and 400mg/mL were tested on the pathogenic bacteria for antibacterial activities using agar well diffusion assay. Streptomycin sulfate was used as positive control while methanol used as negative control. Phytochemical analysis was done by using standard method. The inhibition zone of the extract show positive results such as 1.90cm, 2.00cm, 2.00cm, 2.00cm, 2.00cm, and 1.92cm on concentration 100, 150, 200, 250, 300 and 350mg/ml respectively. Flavonoid, saponin, terpenoid, and tannin were present in Parkia speciosa pod extract. Pyrogallol which is known as antimicrobial chemical compound was identified in Parkia speciosa crude extract using Gas chromatography–Mass spectrum analysis. 2016 Project Paper Report NonPeerReviewed text en http://psasir.upm.edu.my/id/eprint/91543/1/FP%202016%2026%20-%20IR.pdf Kah, Oi Yuan (2016) Antibacterial activity of Parkia speciosa Hassk pod crude extract against Pseudomonas fuscovaginae. [Project Paper Report]
institution UPM IR
collection UPM IR
language English
description Rice is one of the most important food crops that feed almost over 3 billion of people worldwide. However, the outbreak of pest and diseases affected the world rice production. One of the serious infection on paddy plant is Bacterial Sheath Brown Rot caused by Pseudomonas fuscovaginae. Pseudomonas fuscovaginae is a florescent gram negative pathogenic bacterium. Several control methods had been carried out and found to be effective toward Pseudomonas fuscovaginae yet does not completely eliminate the pathogen from the plant. Parkia speciosa Hassk has been used as folk medicine to treat various human diseases and sickness. Alkaloid, Saponin, Terpenoids, Phenolic, Flavonoid, and Tannin were the phytochemical substances that can be found in all parts of Parkia speciosa plant. Several research had found that methanolic extract of Parkia speciosa pod can inhibit the growth of bacteria Staphylococcus aureus and Escherichia coli. However, there is no research on antibacterial activities of this plant extract toward plant pathogenic bacteria. This study is to identify the antibacterial activities of Parkia speciosa pod extract toward Pseudomonas fuscovaginae and to determine the active chemical compounds in the Parkia speciosa pod. Methanolic pod extract of Parkia speciosa with concentration of 25, 50, 100, 150, 200,250, 300, 350, and 400mg/mL were tested on the pathogenic bacteria for antibacterial activities using agar well diffusion assay. Streptomycin sulfate was used as positive control while methanol used as negative control. Phytochemical analysis was done by using standard method. The inhibition zone of the extract show positive results such as 1.90cm, 2.00cm, 2.00cm, 2.00cm, 2.00cm, and 1.92cm on concentration 100, 150, 200, 250, 300 and 350mg/ml respectively. Flavonoid, saponin, terpenoid, and tannin were present in Parkia speciosa pod extract. Pyrogallol which is known as antimicrobial chemical compound was identified in Parkia speciosa crude extract using Gas chromatography–Mass spectrum analysis.
format Project Paper Report
author Kah, Oi Yuan
spellingShingle Kah, Oi Yuan
Antibacterial activity of Parkia speciosa Hassk pod crude extract against Pseudomonas fuscovaginae
author_facet Kah, Oi Yuan
author_sort Kah, Oi Yuan
title Antibacterial activity of Parkia speciosa Hassk pod crude extract against Pseudomonas fuscovaginae
title_short Antibacterial activity of Parkia speciosa Hassk pod crude extract against Pseudomonas fuscovaginae
title_full Antibacterial activity of Parkia speciosa Hassk pod crude extract against Pseudomonas fuscovaginae
title_fullStr Antibacterial activity of Parkia speciosa Hassk pod crude extract against Pseudomonas fuscovaginae
title_full_unstemmed Antibacterial activity of Parkia speciosa Hassk pod crude extract against Pseudomonas fuscovaginae
title_sort antibacterial activity of parkia speciosa hassk pod crude extract against pseudomonas fuscovaginae
publishDate 2016
url http://psasir.upm.edu.my/id/eprint/91543/1/FP%202016%2026%20-%20IR.pdf
_version_ 1819300519138557952
score 13.4562235

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