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Corrigendum: mitigation of sodium iodate-induced cytotoxicity in retinal pigment epithelial cells in vitro by transgenic erythropoietin-expressing mesenchymal stem cells

Mesenchymal stem cells (MSC) have shown promise in restoring the vision of patients in clinical trials. However, this therapeutic effect is not observed in every treated patient and is possibly due to the inefficacies of cell delivery and high cell death following transplantation. Utilizing erythrop...

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Main Authors: Koh, Avin Ee Hwan, Subbiah, Suresh Kumar, Aisha Farhana, Alam, Mohammad Khursheed, Mok, Pooi Ling
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Published: Frontiers Media 2021
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spelling oai:psasir.upm.edu.my:94206 http://psasir.upm.edu.my/id/eprint/94206/ Corrigendum: mitigation of sodium iodate-induced cytotoxicity in retinal pigment epithelial cells in vitro by transgenic erythropoietin-expressing mesenchymal stem cells Koh, Avin Ee Hwan Subbiah, Suresh Kumar Aisha Farhana Alam, Mohammad Khursheed Mok, Pooi Ling Mesenchymal stem cells (MSC) have shown promise in restoring the vision of patients in clinical trials. However, this therapeutic effect is not observed in every treated patient and is possibly due to the inefficacies of cell delivery and high cell death following transplantation. Utilizing erythropoietin can significantly enhance the regenerative properties of MSCs and hence improve retinal neuron survivability in oxidative stress. Hence, this study aimed to investigate the efficacy of conditioned medium (CM) obtained from transgenic human erythropoietin-expressing MSCs (MSCEPO) in protecting human retinal pigment epithelial cells from sodium iodate (NaIO3)-induced cell death. Human MSC and MSCEPO were first cultured to obtain conditioned media (CM). The IC50 of NaIO3 in the ARPE-19 culture was then determined by an MTT assay. After that, the efficacy of both MSC-CM and MSC-CMEPO in ARPE-19 cell survival were compared at 24 and 48 h after NaIO3 treatment with MTT. The treatment effects on mitochondrial membrane potential was then measured by a JC-1 flow cytometric assay. The MTT results indicated a corresponding increase in cell survivability (5–58%) in the ARPE-19 cell cultures. In comparison to MSC-CM, the use of conditioned medium collected from the MSC-CMEPO further enhanced the rate of ARPE-19 survivability at 24 h (P < 0.05) and 48 h (P < 0.05) in the presence of NaIO3. Furthermore, more than 90% were found viable with the JC-1 assay after MSC-CMEPO treatment, showing a positive implication on the mitochondrial dynamics of ARPE-19. The MSC-CMEPO provided an enhanced mitigating effect against NaIO3-induced ARPE-19 cell death over that of MSC-CM alone during the early phase of the treatment, and it may act as a future therapy in treating retinal degenerative diseases. Frontiers Media 2021-04-15 Article PeerReviewed Koh, Avin Ee Hwan and Subbiah, Suresh Kumar and Aisha Farhana and Alam, Mohammad Khursheed and Mok, Pooi Ling (2021) Corrigendum: mitigation of sodium iodate-induced cytotoxicity in retinal pigment epithelial cells in vitro by transgenic erythropoietin-expressing mesenchymal stem cells. Frontiers in Cell and Developmental Biology, 9. art. no. 652065. pp. 1-9. ISSN 2296-634X https://www.frontiersin.org/articles/10.3389/fcell.2021.652065/full 10.3389/fcell.2021.652065
institution UPM IR
collection UPM IR
description Mesenchymal stem cells (MSC) have shown promise in restoring the vision of patients in clinical trials. However, this therapeutic effect is not observed in every treated patient and is possibly due to the inefficacies of cell delivery and high cell death following transplantation. Utilizing erythropoietin can significantly enhance the regenerative properties of MSCs and hence improve retinal neuron survivability in oxidative stress. Hence, this study aimed to investigate the efficacy of conditioned medium (CM) obtained from transgenic human erythropoietin-expressing MSCs (MSCEPO) in protecting human retinal pigment epithelial cells from sodium iodate (NaIO3)-induced cell death. Human MSC and MSCEPO were first cultured to obtain conditioned media (CM). The IC50 of NaIO3 in the ARPE-19 culture was then determined by an MTT assay. After that, the efficacy of both MSC-CM and MSC-CMEPO in ARPE-19 cell survival were compared at 24 and 48 h after NaIO3 treatment with MTT. The treatment effects on mitochondrial membrane potential was then measured by a JC-1 flow cytometric assay. The MTT results indicated a corresponding increase in cell survivability (5–58%) in the ARPE-19 cell cultures. In comparison to MSC-CM, the use of conditioned medium collected from the MSC-CMEPO further enhanced the rate of ARPE-19 survivability at 24 h (P < 0.05) and 48 h (P < 0.05) in the presence of NaIO3. Furthermore, more than 90% were found viable with the JC-1 assay after MSC-CMEPO treatment, showing a positive implication on the mitochondrial dynamics of ARPE-19. The MSC-CMEPO provided an enhanced mitigating effect against NaIO3-induced ARPE-19 cell death over that of MSC-CM alone during the early phase of the treatment, and it may act as a future therapy in treating retinal degenerative diseases.
format Article
author Koh, Avin Ee Hwan
Subbiah, Suresh Kumar
Aisha Farhana
Alam, Mohammad Khursheed
Mok, Pooi Ling
spellingShingle Koh, Avin Ee Hwan
Subbiah, Suresh Kumar
Aisha Farhana
Alam, Mohammad Khursheed
Mok, Pooi Ling
Corrigendum: mitigation of sodium iodate-induced cytotoxicity in retinal pigment epithelial cells in vitro by transgenic erythropoietin-expressing mesenchymal stem cells
author_facet Koh, Avin Ee Hwan
Subbiah, Suresh Kumar
Aisha Farhana
Alam, Mohammad Khursheed
Mok, Pooi Ling
author_sort Koh, Avin Ee Hwan
title Corrigendum: mitigation of sodium iodate-induced cytotoxicity in retinal pigment epithelial cells in vitro by transgenic erythropoietin-expressing mesenchymal stem cells
title_short Corrigendum: mitigation of sodium iodate-induced cytotoxicity in retinal pigment epithelial cells in vitro by transgenic erythropoietin-expressing mesenchymal stem cells
title_full Corrigendum: mitigation of sodium iodate-induced cytotoxicity in retinal pigment epithelial cells in vitro by transgenic erythropoietin-expressing mesenchymal stem cells
title_fullStr Corrigendum: mitigation of sodium iodate-induced cytotoxicity in retinal pigment epithelial cells in vitro by transgenic erythropoietin-expressing mesenchymal stem cells
title_full_unstemmed Corrigendum: mitigation of sodium iodate-induced cytotoxicity in retinal pigment epithelial cells in vitro by transgenic erythropoietin-expressing mesenchymal stem cells
title_sort corrigendum: mitigation of sodium iodate-induced cytotoxicity in retinal pigment epithelial cells in vitro by transgenic erythropoietin-expressing mesenchymal stem cells
publisher Frontiers Media
publishDate 2021
_version_ 1782761309525245952
score 12.933938

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