Anti-cancer and anti-inflammatory effects elicited by short chain fatty acids produced by Escherichia coli isolated from healthy human gut microbiota

Background: Extracellular metabolites of short chain fatty acids (SCFA) excreted by gut microbiota have been reported to play an important role in the regulation of intestinal homeostasis. Apart from supplying energy, SCFA also elicit immune stimulation in animal and human cells. Therefore, an attem...

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Main Authors: Nakkarach, Atchareeya, Foo, Hooi Ling, Song, Adelene Ai Lian, Abdul Mutalib, Nur Elina, Nitisinprasert, Sunee, Withayagiat, Ulaiwan
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Publicado em: BMC 2021
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spelling oai:psasir.upm.edu.my:95900 http://psasir.upm.edu.my/id/eprint/95900/ Anti-cancer and anti-inflammatory effects elicited by short chain fatty acids produced by Escherichia coli isolated from healthy human gut microbiota Nakkarach, Atchareeya Foo, Hooi Ling Song, Adelene Ai Lian Abdul Mutalib, Nur Elina Nitisinprasert, Sunee Withayagiat, Ulaiwan Background: Extracellular metabolites of short chain fatty acids (SCFA) excreted by gut microbiota have been reported to play an important role in the regulation of intestinal homeostasis. Apart from supplying energy, SCFA also elicit immune stimulation in animal and human cells. Therefore, an attempt was conducted to isolate SCFA producing bacteria from healthy human microbiota. The anti-cancer and anti-inflammatory effects of extracellular metabolites and individual SFCA were further investigated by using breast, colon cancer and macrophage cells. Toxin, inflammatory and anti-inflammatory cytokine gene expressions were investigated by RT-qPCR analyses in this study. Results: Escherichia coli KUB-36 was selected in this study since it has the capability to produce seven SCFA extracellularly. It produced acetic acid as the main SCFA. It is a non-exotoxin producer and hence, it is a safe gut microbiota. The IC50 values indicated that the E. coli KUB-36 metabolites treatment elicited more potent cytotoxicity effect on MCF7 breast cancer cell as compared to colon cancer and leukemia cancer cells but exhibited little cytotoxic effects on normal breast cell. Furthermore, E. coli KUB-36 metabolites and individual SCFA could affect inflammatory responses in lipopolysaccharide-induced THP-1 macrophage cells since they suppressed inflammatory cytokines IL-1β, IL-6, IL-8 and TNF-α well as compared to the control, whilst inducing anti-inflammatory cytokine IL-10 expression. Conclusion: SCFA producing E. coli KUB-36 possessed vast potential as a beneficial gut microbe since it is a non-exotoxin producer that exhibited beneficial cytotoxic effects on cancer cells and elicited anti-inflammatory activity simultaneously. However, the probiotic characteristic of E. coli KUB-36 should be further elucidated using in vivo animal models. BMC 2021 Article PeerReviewed Nakkarach, Atchareeya and Foo, Hooi Ling and Song, Adelene Ai Lian and Abdul Mutalib, Nur Elina and Nitisinprasert, Sunee and Withayagiat, Ulaiwan (2021) Anti-cancer and anti-inflammatory effects elicited by short chain fatty acids produced by Escherichia coli isolated from healthy human gut microbiota. Microbial Cell Factories, 20. art. no. 36. pp. 1-17. ISSN 1475-2859 https://microbialcellfactories.biomedcentral.com/articles/10.1186/s12934-020-01477-z 10.1186/s12934-020-01477-z
institution UPM IR
collection UPM IR
description Background: Extracellular metabolites of short chain fatty acids (SCFA) excreted by gut microbiota have been reported to play an important role in the regulation of intestinal homeostasis. Apart from supplying energy, SCFA also elicit immune stimulation in animal and human cells. Therefore, an attempt was conducted to isolate SCFA producing bacteria from healthy human microbiota. The anti-cancer and anti-inflammatory effects of extracellular metabolites and individual SFCA were further investigated by using breast, colon cancer and macrophage cells. Toxin, inflammatory and anti-inflammatory cytokine gene expressions were investigated by RT-qPCR analyses in this study. Results: Escherichia coli KUB-36 was selected in this study since it has the capability to produce seven SCFA extracellularly. It produced acetic acid as the main SCFA. It is a non-exotoxin producer and hence, it is a safe gut microbiota. The IC50 values indicated that the E. coli KUB-36 metabolites treatment elicited more potent cytotoxicity effect on MCF7 breast cancer cell as compared to colon cancer and leukemia cancer cells but exhibited little cytotoxic effects on normal breast cell. Furthermore, E. coli KUB-36 metabolites and individual SCFA could affect inflammatory responses in lipopolysaccharide-induced THP-1 macrophage cells since they suppressed inflammatory cytokines IL-1β, IL-6, IL-8 and TNF-α well as compared to the control, whilst inducing anti-inflammatory cytokine IL-10 expression. Conclusion: SCFA producing E. coli KUB-36 possessed vast potential as a beneficial gut microbe since it is a non-exotoxin producer that exhibited beneficial cytotoxic effects on cancer cells and elicited anti-inflammatory activity simultaneously. However, the probiotic characteristic of E. coli KUB-36 should be further elucidated using in vivo animal models.
format Article
author Nakkarach, Atchareeya
Foo, Hooi Ling
Song, Adelene Ai Lian
Abdul Mutalib, Nur Elina
Nitisinprasert, Sunee
Withayagiat, Ulaiwan
spellingShingle Nakkarach, Atchareeya
Foo, Hooi Ling
Song, Adelene Ai Lian
Abdul Mutalib, Nur Elina
Nitisinprasert, Sunee
Withayagiat, Ulaiwan
Anti-cancer and anti-inflammatory effects elicited by short chain fatty acids produced by Escherichia coli isolated from healthy human gut microbiota
author_facet Nakkarach, Atchareeya
Foo, Hooi Ling
Song, Adelene Ai Lian
Abdul Mutalib, Nur Elina
Nitisinprasert, Sunee
Withayagiat, Ulaiwan
author_sort Nakkarach, Atchareeya
title Anti-cancer and anti-inflammatory effects elicited by short chain fatty acids produced by Escherichia coli isolated from healthy human gut microbiota
title_short Anti-cancer and anti-inflammatory effects elicited by short chain fatty acids produced by Escherichia coli isolated from healthy human gut microbiota
title_full Anti-cancer and anti-inflammatory effects elicited by short chain fatty acids produced by Escherichia coli isolated from healthy human gut microbiota
title_fullStr Anti-cancer and anti-inflammatory effects elicited by short chain fatty acids produced by Escherichia coli isolated from healthy human gut microbiota
title_full_unstemmed Anti-cancer and anti-inflammatory effects elicited by short chain fatty acids produced by Escherichia coli isolated from healthy human gut microbiota
title_sort anti-cancer and anti-inflammatory effects elicited by short chain fatty acids produced by escherichia coli isolated from healthy human gut microbiota
publisher BMC
publishDate 2021
_version_ 1819300813738082304
score 13.4562235