Imagen de Portada

Evaluation of Tween 80 incorporated media to increase pathogen isolation from peritoneal fluid of CAPD patients at Hospital Kuala Lumpur

Introduction: Continuous ambulatory peritoneal dialysis (CAPD)-associated peritonitis remains a major complication in patients on CAPD leading to increased morbidity and mortality. Successful therapy of peritonitis is highly dependent on a positive microbiological culture because narrow spectrum ant...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores Principales: Ganapathy Pillay, Stella, Mohd Amin, Sofia Duratul Waheda, Masri, Siti Norbaya, Sither Joseph Vesudian, Narcisse Mary, Amran, Fairuz, Van Belkum, Alex, Amin Nordin, Syafinaz
Formato: Artículo
Publicado: Malaysian Society of Pathologists 2021
Etiquetas: Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
id oai:psasir.upm.edu.my:97179
record_format eprints
spelling oai:psasir.upm.edu.my:97179 http://psasir.upm.edu.my/id/eprint/97179/ Evaluation of Tween 80 incorporated media to increase pathogen isolation from peritoneal fluid of CAPD patients at Hospital Kuala Lumpur Ganapathy Pillay, Stella Mohd Amin, Sofia Duratul Waheda Masri, Siti Norbaya Sither Joseph Vesudian, Narcisse Mary Amran, Fairuz Van Belkum, Alex Amin Nordin, Syafinaz Introduction: Continuous ambulatory peritoneal dialysis (CAPD)-associated peritonitis remains a major complication in patients on CAPD leading to increased morbidity and mortality. Successful therapy of peritonitis is highly dependent on a positive microbiological culture because narrow spectrum antibiotics are essential to efficiently combat infection. Therefore, this study evaluated the performance of Tween 80 containing media at three different concentrations (0.1%, 1.0% and 2.0%) to increase the pathogen yield from peritoneal fluid in comparison with the standard culture media. Materials and methods: Peritoneal fluid samples (n=121) obtained from CAPD patients suspected of peritonitis at Hospital Kuala Lumpur were analysed macroscopically and microscopically prior to culture. All samples were cultured on seven different culture media, including sheep blood agar, MacConkey agar, Sabouraud dextrose agar, brain heart infusion agar and Tween 80 incorporated blood agar. All plates were incubated at an optimum temperature up to 48 hours. Results and conclusion: Among all the culture media investigated, 0.1% to 2.0% Tween 80 incorporated blood agar yielded the highest positive culture (23/121) in comparison with all other standard media, thus lowering the negative culture rate among CAPD patients. Statistical analysis by Chi Square revealed significant differences (p <0.001) between the three concentrations of Tween 80 tested in this study. Among the three different concentrations of Tween 80 optimised in this study, blood agar containing 0.1% Tween 80 generated the best results, achieved by optimum growth of all Gram-positive organisms, Gram-negative organisms and yeast cells simultaneously. Using a small amount of detergent at low cost significantly increased the pathogen yield during CAPD-associated peritonitis. Malaysian Society of Pathologists 2021 Article PeerReviewed Ganapathy Pillay, Stella and Mohd Amin, Sofia Duratul Waheda and Masri, Siti Norbaya and Sither Joseph Vesudian, Narcisse Mary and Amran, Fairuz and Van Belkum, Alex and Amin Nordin, Syafinaz (2021) Evaluation of Tween 80 incorporated media to increase pathogen isolation from peritoneal fluid of CAPD patients at Hospital Kuala Lumpur. Malaysian Journal of Pathology, 43 (2). 261 - 268. ISSN 0126-8635 http://www.mjpath.org.my/2021/v43n2/index.php
institution UPM IR
collection UPM IR
description Introduction: Continuous ambulatory peritoneal dialysis (CAPD)-associated peritonitis remains a major complication in patients on CAPD leading to increased morbidity and mortality. Successful therapy of peritonitis is highly dependent on a positive microbiological culture because narrow spectrum antibiotics are essential to efficiently combat infection. Therefore, this study evaluated the performance of Tween 80 containing media at three different concentrations (0.1%, 1.0% and 2.0%) to increase the pathogen yield from peritoneal fluid in comparison with the standard culture media. Materials and methods: Peritoneal fluid samples (n=121) obtained from CAPD patients suspected of peritonitis at Hospital Kuala Lumpur were analysed macroscopically and microscopically prior to culture. All samples were cultured on seven different culture media, including sheep blood agar, MacConkey agar, Sabouraud dextrose agar, brain heart infusion agar and Tween 80 incorporated blood agar. All plates were incubated at an optimum temperature up to 48 hours. Results and conclusion: Among all the culture media investigated, 0.1% to 2.0% Tween 80 incorporated blood agar yielded the highest positive culture (23/121) in comparison with all other standard media, thus lowering the negative culture rate among CAPD patients. Statistical analysis by Chi Square revealed significant differences (p <0.001) between the three concentrations of Tween 80 tested in this study. Among the three different concentrations of Tween 80 optimised in this study, blood agar containing 0.1% Tween 80 generated the best results, achieved by optimum growth of all Gram-positive organisms, Gram-negative organisms and yeast cells simultaneously. Using a small amount of detergent at low cost significantly increased the pathogen yield during CAPD-associated peritonitis.
format Article
author Ganapathy Pillay, Stella
Mohd Amin, Sofia Duratul Waheda
Masri, Siti Norbaya
Sither Joseph Vesudian, Narcisse Mary
Amran, Fairuz
Van Belkum, Alex
Amin Nordin, Syafinaz
spellingShingle Ganapathy Pillay, Stella
Mohd Amin, Sofia Duratul Waheda
Masri, Siti Norbaya
Sither Joseph Vesudian, Narcisse Mary
Amran, Fairuz
Van Belkum, Alex
Amin Nordin, Syafinaz
Evaluation of Tween 80 incorporated media to increase pathogen isolation from peritoneal fluid of CAPD patients at Hospital Kuala Lumpur
author_facet Ganapathy Pillay, Stella
Mohd Amin, Sofia Duratul Waheda
Masri, Siti Norbaya
Sither Joseph Vesudian, Narcisse Mary
Amran, Fairuz
Van Belkum, Alex
Amin Nordin, Syafinaz
author_sort Ganapathy Pillay, Stella
title Evaluation of Tween 80 incorporated media to increase pathogen isolation from peritoneal fluid of CAPD patients at Hospital Kuala Lumpur
title_short Evaluation of Tween 80 incorporated media to increase pathogen isolation from peritoneal fluid of CAPD patients at Hospital Kuala Lumpur
title_full Evaluation of Tween 80 incorporated media to increase pathogen isolation from peritoneal fluid of CAPD patients at Hospital Kuala Lumpur
title_fullStr Evaluation of Tween 80 incorporated media to increase pathogen isolation from peritoneal fluid of CAPD patients at Hospital Kuala Lumpur
title_full_unstemmed Evaluation of Tween 80 incorporated media to increase pathogen isolation from peritoneal fluid of CAPD patients at Hospital Kuala Lumpur
title_sort evaluation of tween 80 incorporated media to increase pathogen isolation from peritoneal fluid of capd patients at hospital kuala lumpur
publisher Malaysian Society of Pathologists
publishDate 2021
_version_ 1819300911078440960
score 13.4562235

Ejemplares similares