Peptide Nucleic Acids Methods and Protocols /
Peptide nucleic acid (PNA) technology continues to grow in importance throughout molecular biology, genetic diagnostics, and molecular medicine. In Peptide Nucleic Acids: Methods and Protocols, Peter Eigil Nielsen has assembled a critically evaluated collection of PNA protocols that are either alrea...
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| Format: | Electronic eBook |
| Language: | English |
| Published: |
Totowa, NJ :
Humana Press : Imprint: Humana,
2002.
|
| Edition: | 1st ed. 2002. |
| Series: | Methods in Molecular Biology,
208 |
| Subjects: | |
| Online Access: | https://doi.org/10.1385/1592592902 |
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| 001 | 978-1-59259-290-6 | ||
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| 245 | 1 | 0 | |a Peptide Nucleic Acids |h [electronic resource] : |b Methods and Protocols / |c edited by Peter E. Nielsen. |
| 250 | |a 1st ed. 2002. | ||
| 264 | 1 | |a Totowa, NJ : |b Humana Press : |b Imprint: Humana, |c 2002. | |
| 300 | |a X, 274 p. |b online resource. | ||
| 336 | |a text |b txt |2 rdacontent | ||
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| 490 | 1 | |a Methods in Molecular Biology, |x 1064-3745 ; |v 208 | |
| 505 | 0 | |a PNA Technology -- Chemistry -- Solid Phase Synthesis of PNA Oligomers -- Synthesis of PNA-Peptide Conjugates -- Parallel Synthesis of PNA-Peptide Conjugate Libraries -- Thermodynamics of PNA Interactions with DNA and RNA -- PNA Technology -- Measurement of PNA Binding to Double-Stranded DNA -- PNA-Mediated Immobilization of Supercoiled DNA -- PNA Openers and Their Applications -- Colorimetric Detection of PNA-DNA Hybridization Using Cyanine Dyes -- Diagnostics -- PNA-Mediated PCR Clamping -- PNA Clamping Techniques for the Determination of Oncogene Mutations -- PNA Fluorescent In Situ Hybridization for Rapid Microbiology and Cytogenetic Analysis -- Detection of Point Mutations Using PNA-Containing Electrophoresis Matrices -- Antisense and Antigene -- Lipid-Mediated Introduction of Peptide Nucleic Acids Into Cells -- Synthesis of Cell-Penetrating Peptide-PNA Constructs -- Antisense Inhibition of Bacterial Gene Expression and Cell Growth -- In Vitro Transcription from Peptide Nucleic Acid/DNA Strand Displacement Loops -- Animal Studies -- In Vitro and In Vivo Studies on the Pharmacokinetics and Metabolism of PNA Constructs in Rodents. | |
| 520 | |a Peptide nucleic acid (PNA) technology continues to grow in importance throughout molecular biology, genetic diagnostics, and molecular medicine. In Peptide Nucleic Acids: Methods and Protocols, Peter Eigil Nielsen has assembled a critically evaluated collection of PNA protocols that are either already well established around the world, such as PCR-clamping and in situ hybridization, or that display promise of significant future impact. Basic methods for PNA oligomer synthesis and analysis have also been included. Written by experts with hands-on experience in the methods they describe, these readily reproducible protocols contain detailed step-by-step instructions, tips on avoiding pitfalls and on extending the method to other situations, and introductory material explaining the theory behind the process. Up-to-date and highly practical, Peptide Nucleic Acids: Methods and Protocols provides for both novice and experienced researchers a thorough and easy introduction to the optimal use of today's PNA technology across a wide variety of fields, including molecular biology, medicinal chemistry, genetic diagnostics, and drug development. | ||
| 650 | 0 | |a Biochemistry. | |
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| 700 | 1 | |a Nielsen, Peter E. |e editor. |4 edt |4 http://id.loc.gov/vocabulary/relators/edt | |
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| 830 | 0 | |a Methods in Molecular Biology, |x 1064-3745 ; |v 208 | |
| 856 | 4 | 0 | |u https://doi.org/10.1385/1592592902 |
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| 950 | |a Springer Protocols (Springer-12345) | ||