Microbial Gene Essentiality: Protocols and Bioinformatics
Microbial Gene Essentiality: Protocols and Bioinformatics contains a comprehensive collection of experimental and computational strategies and techniques for microbial genome-scale essentiality studies, developed and presented by the leading groups in the field. In addition to wet-lab protocols, the...
محفوظ في:
| مؤلف مشترك: | |
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| مؤلفون آخرون: | , |
| التنسيق: | الكتروني كتاب الكتروني |
| اللغة: | English |
| منشور في: |
Totowa, NJ :
Humana Press : Imprint: Humana,
2008.
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| الطبعة: | 1st ed. 2008. |
| سلاسل: | Methods in Molecular Biology,
416 |
| الموضوعات: | |
| الوصول للمادة أونلاين: | https://doi.org/10.1007/978-1-59745-321-9 |
| الوسوم: |
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جدول المحتويات:
- Overview of Whole-Genome Essentiality Analysis
- Experimental Protocols
- Transposon-Based Strategies for the Identification of Essential Bacterial Genes
- Identification and Analysis of Essential Genes in Haemophilus influenzae
- Transposon Site Hybridization in Mycobacterium tuberculosis
- Essential Genes in the Infection Model of Pseudomonas aeruginosa PCR-Based Signature-Tagged Mutagenesis
- Whole-Genome Detection of Conditionally Essential and Dispensable Genes in Escherichia coli via Genetic Footprinting
- Generating a Collection of Insertion Mutations in the Staphylococcus aureus Genome Using bursa aurealis
- Multipurpose Transposon Insertion Libraries for Large-Scale Analysis of Gene Function in Yeast
- How to Make a Defined Near-Saturation Mutant Library. Case 1: Pseudomonas aeruginosa PAO1
- Comparing Insertion Libraries in Two Pseudomonas aeruginosa Strains to Assess Gene Essentiality
- The Construction of Systematic In-Frame, Single-Gene Knockout Mutant Collection in Escherichia coli K-12
- The Applications of Systematic In-Frame, Single-Gene Knockout Mutant Collection of Escherichia coli K-12
- A Novel, Simple, High-Throughput Method for Isolation of Genome-Wide Transposon Insertion Mutants of Escherichia coli K-12
- High-Throughput Creation of a Whole-Genome Collection of Yeast Knockout Strains
- Analysis of Genetic Interactions on a Genome-Wide Scale in Budding Yeast: Diploid-Based Synthetic Lethality Analysis by Microarray
- Scarless Engineering of the Escherichia coli Genome
- Minimization of the Escherichia coli Genome Using the Tn5-Targeted Cre/loxP Excision System
- Construction of Long Chromosomal Deletion Mutants of Escherichia coli and Minimization of the Genome
- Identification of Essential Genes in Staphylococcus aureus by Construction and Screening of Conditional Mutant Library
- Techniques for the Isolation and Use of Conditionally Expressed Antisense RNA to Achieve Essential Gene Knockdowns in Staphylococcus aureus
- of Conditional Lethal Amber Mutations in Escherichia coli
- Bioinformatics
- Statistical Methods for Building Random Transposon Mutagenesis Libraries
- Statistical Evaluation of Genetic Footprinting Data
- Modeling Competitive Outgrowth of Mutant Populations: Why Do Essentiality Screens Yield Divergent Results?
- Statistical Analysis of Fitness Data Determined by TAG Hybridization on Microarrays
- Profiling of Escherichia coli Chromosome Database
- Gene Essentiality Analysis Based on DEG, a Database of Essential Genes
- Detection of Essential Genes in Streptococcus pneumoniae Using Bioinformatics and Allelic Replacement Mutagenesis
- Design and Application of Genome-Scale Reconstructed Metabolic Models
- Predicting Gene Essentiality Using Genome-Scale in Silico Models
- Comparative Approach to Analysis of Gene Essentiality.