RT-PCR Protocols Second Edition /
Once a tedious, highly skilled operation, reverse-transcription polymerase chain reaction (RT-PCR) has become a routine and invaluable technique used in most laboratories. In RT-PCR Protocols, Second Edition, expert researchers fully update the technologies presented in the popular previous edition,...
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| Format: | Electronic eBook |
| Language: | English |
| Published: |
Totowa, NJ :
Humana Press : Imprint: Humana,
2010.
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| Edition: | 2nd ed. 2010. |
| Series: | Methods in Molecular Biology,
630 |
| Subjects: | |
| Online Access: | https://doi.org/10.1007/978-1-60761-629-0 |
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Table of Contents:
- The RT-PCR Detective: Hunting Down the Best Method
- Single Cell RT-PCR on Mouse Embryos: A General Approach for Developmental Biology
- Poly(A) cDNA Real-Time PCR for Indicator Gene Measurement in Cancer
- Transcriptome Profiling of Host–Microbe Interactions by Differential Display RT-PCR
- Quantitative RT-PCR Methods for Mature microRNA Expression Analysis
- Detection of Influenza A Virus Neuraminidase and PB2 Gene Segments by One Step Reverse Transcription Polymerase Chain Reaction
- Detection and Identification of CD46 Splicing Isoforms by Nested RT-PCR
- Simultaneous Detection of Bluetongue Virus RNA, Internal Control GAPDH mRNA, and External Control Synthetic RNA by Multiplex Real-Time PCR
- Detection of West Nile Viral RNA from Field-Collected Mosquitoes in Tropical Regions by Conventional and Real-Time RT-PCR
- Detection of Antisense RNA Transcripts by Strand-Specific RT-PCR
- RT-PCR Amplification and Cloning of Large Viral Sequences
- The RT-PCR Mathematician: Assessing Gene and RNA Expression
- One-Step RT-LATE-PCR for mRNA and Viral RNA Detection and Quantification
- Changes in Gene Expression of Caveolin-1 After Inflammatory Pain Using Quantitative Real-Time PCR
- Real-Time Quantitative Reverse Transcriptase Polymerase Chain Reaction
- The Use of Comparative Quantitative RT-PCR to Investigate the Effect of Cysteine Incubation on GPx1 Expression in Freshly Isolated Cardiomyocytes
- The Renaissance of Competitive PCR as an Accurate Tool for Precise Nucleic Acid Quantification
- The RT-PCR Master Chef: Finding the Right Ingredients
- Skeletal Muscle RNA Extraction in Preparation for RT-PCR
- Reverse Transcription of the Ribonucleic Acid: The First Step in RT-PCR Assay
- Primer Design for RT-PCR
- Hot Start PCR
- Real-time RT-PCR for Automated Detection of HIV-1 RNA During Blood Donor Screening.