Enzyme Engineering Methods and Protocols /
Whether the pursuit is commercially motivated or purely academic, engineering a novel biological catalyst is an enticing challenge. High-resolution protein structure analysis allows for rational alteration of enzyme function, yet many useful enzyme variants are the product of well-designed selection...
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| Altres autors: | |
| Format: | Electrònic eBook |
| Idioma: | English |
| Publicat: |
Totowa, NJ :
Humana Press : Imprint: Humana,
2013.
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| Edició: | 1st ed. 2013. |
| Periòdiques: | Methods in Molecular Biology,
978 |
| Matèries: | |
| Accés en línia: | https://doi.org/10.1007/978-1-62703-293-3 |
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| 001 | 978-1-62703-293-3 | ||
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| 024 | 7 | |a 10.1007/978-1-62703-293-3 |2 doi | |
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| 245 | 1 | 0 | |a Enzyme Engineering |h [electronic resource] : |b Methods and Protocols / |c edited by James C. Samuelson. |
| 250 | |a 1st ed. 2013. | ||
| 264 | 1 | |a Totowa, NJ : |b Humana Press : |b Imprint: Humana, |c 2013. | |
| 300 | |a XI, 252 p. 52 illus., 19 illus. in color. |b online resource. | ||
| 336 | |a text |b txt |2 rdacontent | ||
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| 490 | 1 | |a Methods in Molecular Biology, |x 1064-3745 ; |v 978 | |
| 505 | 0 | |a A Tripartite Fusion System for the Selection of Protein Variants with Increased Stability in vivo -- Determining Enzyme Kinetics via Isothermal Titration Calorimetry -- GFP Reporter Screens for the Engineering of Amino Acid Degrading Enzymes from Libraries Expressed in Bacteria -- Flow Cytometric Assays for Interrogating LAGLIDADG Homing Endonuclease DNA Binding and Cleavage Properties -- TAL Effector Nuclease (TALEN) Engineering -- In vitro Evolution of Enzymes -- Residue-Specific Incorporation of Unnatural Amino Acids into Proteins In vitro and In vivo -- Reconstructing Evolutionary Adaptive Paths for Protein Engineering -- Oligonucleotide Recombination Enabled Site-Specific Mutagenesis in Bacteria -- FX Cloning: A Versatile High-Throughput Cloning System for Characterization of Enzyme Variants -- Use of Sulfolobus solfataricus PCNA Subunit Proteins to Direct the Assembly of Multimeric Enzyme Complexes -- Gene Synthesis by Assembly of Deoxyuridine Containing Oligonucleotides -- Protein Engineering: Single or Multiple Site-Directed Mutagenesis -- Gene Assembly and Combinatorial Libraries in S. cerevisiae via Reiterative Recombination -- Promiscuity-Based Enzyme Selection for Rational Directed Evolution Experiments -- Rational Protein Sequence Diversification by Multi-Codon Scanning Mutagenesis -- Screening Libraries for Improved Solubility: Using E. coli Dihydrofolate Reductase as a Reporter -- In Vitro Directed Evolution of Enzymes Expressed by E. coli in Micro-Titre Plates. | |
| 520 | |a Whether the pursuit is commercially motivated or purely academic, engineering a novel biological catalyst is an enticing challenge. High-resolution protein structure analysis allows for rational alteration of enzyme function, yet many useful enzyme variants are the product of well-designed selection schemes or screening strategies. Enzyme Engineering: Methods and Protocols provides guidance to investigators wishing to create enzyme variants with desired properties. This detailed volume covers such topics as a simple method for generating site-specific mutations within bacterial chromosomes. It also highlights the engineering of two difference types of rare-cutting endonucleases that show great potential in gene therapy applications: The newest development is the emergence of TAL effector nucleases or TALENs. Chapters describe newly developed technologies in sufficient detail so that each method can be practiced in a standard molecular biology laboratory. Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible Enzyme Engineering: Methods and Protocols will be valuable for scientists with a budding interest in protein engineering as well as veterans looking for new approaches to apply in established discovery programs. | ||
| 650 | 0 | |a Biochemistry. | |
| 650 | 0 | |a Enzymology. | |
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| 650 | 2 | 4 | |a Enzymology. |0 https://scigraph.springernature.com/ontologies/product-market-codes/L14070 |
| 700 | 1 | |a Samuelson, James C. |e editor. |4 edt |4 http://id.loc.gov/vocabulary/relators/edt | |
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| 773 | 0 | |t Springer Nature eBook | |
| 776 | 0 | 8 | |i Printed edition: |z 9781627032940 |
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| 830 | 0 | |a Methods in Molecular Biology, |x 1064-3745 ; |v 978 | |
| 856 | 4 | 0 | |u https://doi.org/10.1007/978-1-62703-293-3 |
| 912 | |a ZDB-2-PRO | ||
| 950 | |a Springer Protocols (Springer-12345) | ||