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Comparison of bovine embryonic development of parthenogenetically activated oocytes by different combinations of chemical treatments

Oocyte activation is the efficient method or step for successful animal cloning. This study was carried out to evaluate the embryonic development for both parthenotes and fertilised embryos according to the oocyte grades (Grades A, B and C). Recovered oocytes according to the oocyte grades were divi...

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Main Authors: Siti Khadijah, I., Rahman, M. M., Abdullah, R. B., Wan Khadijah, W. E.
Format: Journal Contribution
Language:English
Published: 2016
Subjects:
Cloning
Parthenogenesis
Bovines
Oocytes
Chemical analysis (methods)
Embryonic development
Online Access:http://agris.upm.edu.my:8080/dspace/handle/0/10795
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spelling oai:http:--agris.upm.edu.my:0-10795Comparison of bovine embryonic development of parthenogenetically activated oocytes by different combinations of chemical treatmentsSiti Khadijah, I.Rahman, M. M.Abdullah, R. B.Wan Khadijah, W. E.CloningParthenogenesisBovinesOocytesChemical analysis (methods)Embryonic developmentOocyte activation is the efficient method or step for successful animal cloning. This study was carried out to evaluate the embryonic development for both parthenotes and fertilised embryos according to the oocyte grades (Grades A, B and C). Recovered oocytes according to the oocyte grades were divided into various combination treatments: (i) A23187+6-DMAP, (ii) Iono+6-DMAP, (iii) EtOH+6-DMAP, (iv) Iono+CHX, and (v) IVF (control). Treatment with combination of 10 μM Iono+2 mM 6-DMAP indicated the highest embryonic development rates for 8-cell, morula and blastocyst stages compared to the other combination treatments. There were significant differences between combination of Iono+6-DMAP and IVF control at morula (24.3 vs. 3.4%) and blastocyst stages (7.1 vs. 0.0%), respectively. There was no consistent relationship between oocyte grades and embryo development rates. The data also showed that no differences were observed in blastocyst rates for each oocyte grade for all activation treatments including IVF control. In conclusion, to activate bovine oocytes for later embryonic development stages (8-cell, morula and blastocyst), the best combination treatment obtained in this study was the combination of 10 μM Iono (5 min)+2 mM 6-DMAP (4 h), regardless of the grades of oocytes, including the importance of the optimal combination of chemical activation to produce bovine embryos in vitro.2016-02-18T07:00:02Z2016-02-18T07:00:02Z2014Journal ContributionArticleNon-RefereedMalaysian Journal Of Animal Science (Malaysia), 17 (1), p. 85-941394-3227http://agris.upm.edu.my:8080/dspace/handle/0/10795MY2016050078enhttp://www.msap.my/pdf/8-Comparison-Ramli_rev7.pdfMalaysiahttp://www.oceandocs.org/license
institution AGRIS
collection AGRIS
language English
topic Cloning
Parthenogenesis
Bovines
Oocytes
Chemical analysis (methods)
Embryonic development
spellingShingle Cloning
Parthenogenesis
Bovines
Oocytes
Chemical analysis (methods)
Embryonic development
Siti Khadijah, I.
Rahman, M. M.
Abdullah, R. B.
Wan Khadijah, W. E.
Comparison of bovine embryonic development of parthenogenetically activated oocytes by different combinations of chemical treatments
description Oocyte activation is the efficient method or step for successful animal cloning. This study was carried out to evaluate the embryonic development for both parthenotes and fertilised embryos according to the oocyte grades (Grades A, B and C). Recovered oocytes according to the oocyte grades were divided into various combination treatments: (i) A23187+6-DMAP, (ii) Iono+6-DMAP, (iii) EtOH+6-DMAP, (iv) Iono+CHX, and (v) IVF (control). Treatment with combination of 10 μM Iono+2 mM 6-DMAP indicated the highest embryonic development rates for 8-cell, morula and blastocyst stages compared to the other combination treatments. There were significant differences between combination of Iono+6-DMAP and IVF control at morula (24.3 vs. 3.4%) and blastocyst stages (7.1 vs. 0.0%), respectively. There was no consistent relationship between oocyte grades and embryo development rates. The data also showed that no differences were observed in blastocyst rates for each oocyte grade for all activation treatments including IVF control. In conclusion, to activate bovine oocytes for later embryonic development stages (8-cell, morula and blastocyst), the best combination treatment obtained in this study was the combination of 10 μM Iono (5 min)+2 mM 6-DMAP (4 h), regardless of the grades of oocytes, including the importance of the optimal combination of chemical activation to produce bovine embryos in vitro.
format Journal Contribution
author Siti Khadijah, I.
Rahman, M. M.
Abdullah, R. B.
Wan Khadijah, W. E.
author_facet Siti Khadijah, I.
Rahman, M. M.
Abdullah, R. B.
Wan Khadijah, W. E.
author_sort Siti Khadijah, I.
title Comparison of bovine embryonic development of parthenogenetically activated oocytes by different combinations of chemical treatments
title_short Comparison of bovine embryonic development of parthenogenetically activated oocytes by different combinations of chemical treatments
title_full Comparison of bovine embryonic development of parthenogenetically activated oocytes by different combinations of chemical treatments
title_fullStr Comparison of bovine embryonic development of parthenogenetically activated oocytes by different combinations of chemical treatments
title_full_unstemmed Comparison of bovine embryonic development of parthenogenetically activated oocytes by different combinations of chemical treatments
title_sort comparison of bovine embryonic development of parthenogenetically activated oocytes by different combinations of chemical treatments
publishDate 2016
url http://agris.upm.edu.my:8080/dspace/handle/0/10795
_version_ 1819284707462873088
score 13.4562235

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