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Effect of pre-slaughter stunning on the death of the poultry and myofiber apoptosis

The effectiveness of poultry stunning in producing swift slaughtering was analysed in response to the time needed for the chickens to become insensible upon neck cutting (Td) and the induction of myofiber apoptosis. In total, 49 chicken broilers (BW of 2.17 ± .24 kg) were sacrificed with pre-slaught...

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Main Authors: Shahdan, I. A., Rahman, M. T.
Format: Journal Contribution
Language:English
Published: 2016
Subjects:
Poultry
Slaughtering
Stunning
Apoptosis
Online Access:http://agris.upm.edu.my:8080/dspace/handle/0/11221
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spelling oai:http:--agris.upm.edu.my:0-11221Effect of pre-slaughter stunning on the death of the poultry and myofiber apoptosisShahdan, I. A.Rahman, M. T.PoultrySlaughteringStunningApoptosisThe effectiveness of poultry stunning in producing swift slaughtering was analysed in response to the time needed for the chickens to become insensible upon neck cutting (Td) and the induction of myofiber apoptosis. In total, 49 chicken broilers (BW of 2.17 ± .24 kg) were sacrificed with pre-slaughter stunning, using a constant voltage stunner where the electric current varied between 7.2 to 124.3 mA, and without stunning. The electric current applied during stunning was found to have no effect on Td. Number of apoptotic myonuclei did not vary among stunned and unstunned meat. Apoptosis inducing factor (AIF) and caspase 3 expressions were also not detected in the meat samples of both stunned and unstunned groups at 1 d postmortem. Since the slaughtering process and stunning are associated with stress, the expression of 70 kDa-heat shock protein (Hsp70) was investigated. Moreover Hsp70 is also an inhibitor of apoptosis, by preventing the activation of AIF and apoptosome which stimulates caspase 3 activation. However, expression of Hsp70 was not induced in both stunned groups and unstunned groups. Together, this study found that poultry stunning does not affect Td and myofiber apoptosis.2016-05-13T04:28:15Z2016-05-13T04:28:15Z2014Journal ContributionArticleNon-RefereedInternational Food Research Journal (Malaysia), 21 (6), p. 2279-228422317546http://agris.upm.edu.my:8080/dspace/handle/0/11221MY2016050381enhttp://ifrj.upm.edu.my/21%20(06)%202014/31%20IFRJ%2021%20(06)%202014%20Intan%20083.pdfMalaysiahttp://www.oceandocs.org/license
institution AGRIS
collection AGRIS
language English
topic Poultry
Slaughtering
Stunning
Apoptosis
spellingShingle Poultry
Slaughtering
Stunning
Apoptosis
Shahdan, I. A.
Rahman, M. T.
Effect of pre-slaughter stunning on the death of the poultry and myofiber apoptosis
description The effectiveness of poultry stunning in producing swift slaughtering was analysed in response to the time needed for the chickens to become insensible upon neck cutting (Td) and the induction of myofiber apoptosis. In total, 49 chicken broilers (BW of 2.17 ± .24 kg) were sacrificed with pre-slaughter stunning, using a constant voltage stunner where the electric current varied between 7.2 to 124.3 mA, and without stunning. The electric current applied during stunning was found to have no effect on Td. Number of apoptotic myonuclei did not vary among stunned and unstunned meat. Apoptosis inducing factor (AIF) and caspase 3 expressions were also not detected in the meat samples of both stunned and unstunned groups at 1 d postmortem. Since the slaughtering process and stunning are associated with stress, the expression of 70 kDa-heat shock protein (Hsp70) was investigated. Moreover Hsp70 is also an inhibitor of apoptosis, by preventing the activation of AIF and apoptosome which stimulates caspase 3 activation. However, expression of Hsp70 was not induced in both stunned groups and unstunned groups. Together, this study found that poultry stunning does not affect Td and myofiber apoptosis.
format Journal Contribution
author Shahdan, I. A.
Rahman, M. T.
author_facet Shahdan, I. A.
Rahman, M. T.
author_sort Shahdan, I. A.
title Effect of pre-slaughter stunning on the death of the poultry and myofiber apoptosis
title_short Effect of pre-slaughter stunning on the death of the poultry and myofiber apoptosis
title_full Effect of pre-slaughter stunning on the death of the poultry and myofiber apoptosis
title_fullStr Effect of pre-slaughter stunning on the death of the poultry and myofiber apoptosis
title_full_unstemmed Effect of pre-slaughter stunning on the death of the poultry and myofiber apoptosis
title_sort effect of pre-slaughter stunning on the death of the poultry and myofiber apoptosis
publishDate 2016
url http://agris.upm.edu.my:8080/dspace/handle/0/11221
_version_ 1819284751643574272
score 13.4562235

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