Staff View: Screening of lactic acid bacteria producing uricase and stability assessment in simulated gastrointestinal conditions

Screening of lactic acid bacteria producing uricase and stability assessment in simulated gastrointestinal conditions

Thirteen probiotic lactic acid bacterial strains were screened for extracellular and intracellular uricase production. Results showed that all the strains could produce uricase with three strains, namely Lactobacillus sp.OL-5, Lactobacillus plantarum Mut-7, and Lactobacillus plantarum Dad-13, produc...

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Main Authors:	Utami, T., Handayani, I., Hidayat, C., Rahayu, E. S.
Format:	Journal Contribution
Language:	English
Published:	2023
Subjects:	Lactic acid bacteria Gastrointestinal system Lactobacillus Oxidation Stability
Online Access:	http://agris.upm.edu.my:8080/dspace/handle/0/23151
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spelling	oai:http:--agris.upm.edu.my:0-23151Screening of lactic acid bacteria producing uricase and stability assessment in simulated gastrointestinal conditionsUtami, T.Handayani, I.Hidayat, C.Rahayu, E. S.Lactic acid bacteriaGastrointestinal systemLactobacillusOxidationStabilityThirteen probiotic lactic acid bacterial strains were screened for extracellular and intracellular uricase production. Results showed that all the strains could produce uricase with three strains, namely Lactobacillus sp.OL-5, Lactobacillus plantarum Mut-7, and Lactobacillus plantarum Dad-13, producing high intracellular uricase activities. Further analysis in simulated gastrointestinal conditions showed that Lactobacillus sp. OL-5, and Lactobacillus plantarum Dad-13 intracellular uricase, remained active after addition of gastric juice and duodenal juice. On the other hand, the intracellular of Lactobacillus plantarum Mut-7, and as well as both extracellular and membrane bond uricases of the three strains were not stable in gastric juice and duodenal juice. Thus based on uricase production and the stability in simulated gastrointestinal system, Lactobacillus plantarum Dad-13 is a potential strain for producing uricase.2023-08-29T09:17:51Z2023-08-29T09:17:51Z2018Journal ContributionArticleNon-RefereedInternational Food Research Journal (Malaysia), 25 (4), p. 1661-16672231-754http://agris.upm.edu.my:8080/dspace/handle/0/23151MY2023051149enhttp://www.ifrj.upm.edu.my/25%20(04)%202018/(45).pdfIndonesiahttp://www.oceandocs.org/license
institution	AGRIS
collection	AGRIS
language	English
topic	Lactic acid bacteria Gastrointestinal system Lactobacillus Oxidation Stability
spellingShingle	Lactic acid bacteria Gastrointestinal system Lactobacillus Oxidation Stability Utami, T. Handayani, I. Hidayat, C. Rahayu, E. S. Screening of lactic acid bacteria producing uricase and stability assessment in simulated gastrointestinal conditions
description	Thirteen probiotic lactic acid bacterial strains were screened for extracellular and intracellular uricase production. Results showed that all the strains could produce uricase with three strains, namely Lactobacillus sp.OL-5, Lactobacillus plantarum Mut-7, and Lactobacillus plantarum Dad-13, producing high intracellular uricase activities. Further analysis in simulated gastrointestinal conditions showed that Lactobacillus sp. OL-5, and Lactobacillus plantarum Dad-13 intracellular uricase, remained active after addition of gastric juice and duodenal juice. On the other hand, the intracellular of Lactobacillus plantarum Mut-7, and as well as both extracellular and membrane bond uricases of the three strains were not stable in gastric juice and duodenal juice. Thus based on uricase production and the stability in simulated gastrointestinal system, Lactobacillus plantarum Dad-13 is a potential strain for producing uricase.
format	Journal Contribution
author	Utami, T. Handayani, I. Hidayat, C. Rahayu, E. S.
author_facet	Utami, T. Handayani, I. Hidayat, C. Rahayu, E. S.
author_sort	Utami, T.
title	Screening of lactic acid bacteria producing uricase and stability assessment in simulated gastrointestinal conditions
title_short	Screening of lactic acid bacteria producing uricase and stability assessment in simulated gastrointestinal conditions
title_full	Screening of lactic acid bacteria producing uricase and stability assessment in simulated gastrointestinal conditions
title_fullStr	Screening of lactic acid bacteria producing uricase and stability assessment in simulated gastrointestinal conditions
title_full_unstemmed	Screening of lactic acid bacteria producing uricase and stability assessment in simulated gastrointestinal conditions
title_sort	screening of lactic acid bacteria producing uricase and stability assessment in simulated gastrointestinal conditions
publishDate	2023
url	http://agris.upm.edu.my:8080/dspace/handle/0/23151
_version_	1783895053926662144
score	12.935284

Screening of lactic acid bacteria producing uricase and stability assessment in simulated gastrointestinal conditions

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