Effect of initial glucose concentration on esters production and growth kinetics of Hansenula anomala
Studies were conducted to determine the optimum condition for the production of esters using Hansenula anomala a yeast, in different concentrations of glucose medium. Liquefaction and saccharification of rice starch were carried out using Kleistase E-5 and amyloglucosidase. Initial concentrations if...
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| Main Authors: | , , , |
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| Format: | Journal Contribution |
| Language: | English |
| Published: |
2014
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| Subjects: | |
| Online Access: | http://agris.upm.edu.my:8080/dspace/handle/0/7160 |
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| Summary: | Studies were conducted to determine the optimum condition for the production of esters using Hansenula anomala a yeast, in different concentrations of glucose medium. Liquefaction and saccharification of rice starch were carried out using Kleistase E-5 and amyloglucosidase. Initial concentrations if 3 percent, 5 percent, 10 percent, 15 percent and 20 percent glucose were used. Changes in ethyl acetate, tributyl acetate and ethyl decanoate, total glucose and biomass were monitored up to six days. Esters were formed either on the first day of the second day at all initial carbon concentrations of glucose. The study showed that initial carbon concentration influenced the production of esters. An initial concentration of 15 percent glucose produced the highest amount of ethyl acetate (2100 ppm) while that of 5 percent produced the highest amount of tributyl acetate (848 ppm). Glucose utilisation varied from 93 percent to 99 percent. There was significant growth inhibition at high concentrations (15 - 20 percent) of glucose probably due to substrate inhitor or the osmotic pressure of the medium. The study of growth kinetics indicated that the biomass was important in ester formation- the amount of esters produced increasing with increasing amounts of biomass. |
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