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Inhibition mechanisms of secretome proteins from Paenibacillus polymyxa Kp10 and Lactococcus lactis Gh1 against methicillin-resistant Staphylococcus aureus and vancomycin-resistant Enterococcus

Objective: To determine the inhibition mechanisms of secretome protein extracted from Paenibacillus polymyxa Kp10 (Kp10) and Lactococcus lactis Gh1 (Gh1) against methicillin-resistant Staphylococcus aureus (MRSA) and vancomycin-resistant Enterococcus (VRE). Methods: The sensitivity and viability of...

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Main Authors: Zainal Baharin, Nurul Hana, Khairil Mokhtar, Nur Fadhilah, Mohd Desa, Mohd Nasir, Dzaraly, Nurul Diana, Muthanna, Abdul Rahman, Yuswan, Mohd Hafis, Abbasiliasi, Sahar, Rahmad, Norasfaliza, Wan Nur Ismah, Wan Ahmad Kamil, Hashim, Amalia Mohd, Mustafa, Shuhaimi
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Published: Wolters Kluwer Medknow Publications 2022
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spelling oai:psasir.upm.edu.my:101922 http://psasir.upm.edu.my/id/eprint/101922/ Inhibition mechanisms of secretome proteins from Paenibacillus polymyxa Kp10 and Lactococcus lactis Gh1 against methicillin-resistant Staphylococcus aureus and vancomycin-resistant Enterococcus Zainal Baharin, Nurul Hana Khairil Mokhtar, Nur Fadhilah Mohd Desa, Mohd Nasir Dzaraly, Nurul Diana Muthanna, Abdul Rahman Yuswan, Mohd Hafis Abbasiliasi, Sahar Rahmad, Norasfaliza Wan Nur Ismah, Wan Ahmad Kamil Hashim, Amalia Mohd Mustafa, Shuhaimi Objective: To determine the inhibition mechanisms of secretome protein extracted from Paenibacillus polymyxa Kp10 (Kp10) and Lactococcus lactis Gh1 (Gh1) against methicillin-resistant Staphylococcus aureus (MRSA) and vancomycin-resistant Enterococcus (VRE). Methods: The sensitivity and viability of MRSA and VRE treated with secretome proteins of Kp10 and Gh1 were determined using minimal inhibitory concentration, minimum bactericidal concentration, and time-to-kill assays. The morphological changes were observed using scanning electron microscopy and transmission electron microscopy. To elucidate the antimicrobial mechanism of secretome protein of Kp10 and Gh1 against MRSA and VRE, 2D gel proteomic analysis using liquid chromatography-mass spectrometry was run by comparing upregulated and downregulated proteins, and the proton motive force study including the efflux of ATP, pH gradient, and the membrane potential study were conducted. Results: MRSA and VRE were sensitive to Kp10 and Gh1 secretome protein extracts and displayed apparent morphological and internal composition changes. Several proteins associated with cellular component functions were either downregulated or upregulated in treated MRSA and VRE by changing the membrane potential gradient. Conclusions: Kp10 and Gh1 secretome proteins reduce the growth of VRE and MRSA by damaging the cell membrane. Cell division, cell wall biosynthesis, and protein synthesis are involved in the inhibition mechanism. Wolters Kluwer Medknow Publications 2022-11 Article PeerReviewed Zainal Baharin, Nurul Hana and Khairil Mokhtar, Nur Fadhilah and Mohd Desa, Mohd Nasir and Dzaraly, Nurul Diana and Muthanna, Abdul Rahman and Yuswan, Mohd Hafis and Abbasiliasi, Sahar and Rahmad, Norasfaliza and Wan Nur Ismah, Wan Ahmad Kamil and Hashim, Amalia Mohd and Mustafa, Shuhaimi (2022) Inhibition mechanisms of secretome proteins from Paenibacillus polymyxa Kp10 and Lactococcus lactis Gh1 against methicillin-resistant Staphylococcus aureus and vancomycin-resistant Enterococcus. Asian Pacific Journal of Tropical Biomedicine, 12 (11). pp. 483-494. ISSN 2221-1691 https://journals.lww.com/aptb/fulltext/2022/12110/inhibition_mechanisms_of_secretome_proteins_from.4.aspx 10.4103/2221-1691.360564
institution UPM IR
collection UPM IR
description Objective: To determine the inhibition mechanisms of secretome protein extracted from Paenibacillus polymyxa Kp10 (Kp10) and Lactococcus lactis Gh1 (Gh1) against methicillin-resistant Staphylococcus aureus (MRSA) and vancomycin-resistant Enterococcus (VRE). Methods: The sensitivity and viability of MRSA and VRE treated with secretome proteins of Kp10 and Gh1 were determined using minimal inhibitory concentration, minimum bactericidal concentration, and time-to-kill assays. The morphological changes were observed using scanning electron microscopy and transmission electron microscopy. To elucidate the antimicrobial mechanism of secretome protein of Kp10 and Gh1 against MRSA and VRE, 2D gel proteomic analysis using liquid chromatography-mass spectrometry was run by comparing upregulated and downregulated proteins, and the proton motive force study including the efflux of ATP, pH gradient, and the membrane potential study were conducted. Results: MRSA and VRE were sensitive to Kp10 and Gh1 secretome protein extracts and displayed apparent morphological and internal composition changes. Several proteins associated with cellular component functions were either downregulated or upregulated in treated MRSA and VRE by changing the membrane potential gradient. Conclusions: Kp10 and Gh1 secretome proteins reduce the growth of VRE and MRSA by damaging the cell membrane. Cell division, cell wall biosynthesis, and protein synthesis are involved in the inhibition mechanism.
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author Zainal Baharin, Nurul Hana
Khairil Mokhtar, Nur Fadhilah
Mohd Desa, Mohd Nasir
Dzaraly, Nurul Diana
Muthanna, Abdul Rahman
Yuswan, Mohd Hafis
Abbasiliasi, Sahar
Rahmad, Norasfaliza
Wan Nur Ismah, Wan Ahmad Kamil
Hashim, Amalia Mohd
Mustafa, Shuhaimi
spellingShingle Zainal Baharin, Nurul Hana
Khairil Mokhtar, Nur Fadhilah
Mohd Desa, Mohd Nasir
Dzaraly, Nurul Diana
Muthanna, Abdul Rahman
Yuswan, Mohd Hafis
Abbasiliasi, Sahar
Rahmad, Norasfaliza
Wan Nur Ismah, Wan Ahmad Kamil
Hashim, Amalia Mohd
Mustafa, Shuhaimi
Inhibition mechanisms of secretome proteins from Paenibacillus polymyxa Kp10 and Lactococcus lactis Gh1 against methicillin-resistant Staphylococcus aureus and vancomycin-resistant Enterococcus
author_facet Zainal Baharin, Nurul Hana
Khairil Mokhtar, Nur Fadhilah
Mohd Desa, Mohd Nasir
Dzaraly, Nurul Diana
Muthanna, Abdul Rahman
Yuswan, Mohd Hafis
Abbasiliasi, Sahar
Rahmad, Norasfaliza
Wan Nur Ismah, Wan Ahmad Kamil
Hashim, Amalia Mohd
Mustafa, Shuhaimi
author_sort Zainal Baharin, Nurul Hana
title Inhibition mechanisms of secretome proteins from Paenibacillus polymyxa Kp10 and Lactococcus lactis Gh1 against methicillin-resistant Staphylococcus aureus and vancomycin-resistant Enterococcus
title_short Inhibition mechanisms of secretome proteins from Paenibacillus polymyxa Kp10 and Lactococcus lactis Gh1 against methicillin-resistant Staphylococcus aureus and vancomycin-resistant Enterococcus
title_full Inhibition mechanisms of secretome proteins from Paenibacillus polymyxa Kp10 and Lactococcus lactis Gh1 against methicillin-resistant Staphylococcus aureus and vancomycin-resistant Enterococcus
title_fullStr Inhibition mechanisms of secretome proteins from Paenibacillus polymyxa Kp10 and Lactococcus lactis Gh1 against methicillin-resistant Staphylococcus aureus and vancomycin-resistant Enterococcus
title_full_unstemmed Inhibition mechanisms of secretome proteins from Paenibacillus polymyxa Kp10 and Lactococcus lactis Gh1 against methicillin-resistant Staphylococcus aureus and vancomycin-resistant Enterococcus
title_sort inhibition mechanisms of secretome proteins from paenibacillus polymyxa kp10 and lactococcus lactis gh1 against methicillin-resistant staphylococcus aureus and vancomycin-resistant enterococcus
publisher Wolters Kluwer Medknow Publications
publishDate 2022
_version_ 1819301307891056640
score 13.4562235

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