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Elucidating the mechanisms underlying the action of kenaf seed peptides mixture against gram-positive and gram-negative bacteria and its efficacy in whole milk preservation

The present study determined the in-situ antibacterial effect of cationic kenaf seed peptides mixture (KSPM) in whole milk and further comprehensively elucidated the mechanisms underlying the bactericidal action against gram-positive and gram-negative bacteria. The application experiments in artific...

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Main Authors: Arulrajah, Brisha, Qoms, Mohammed S., Muhialdin, Belal J., Meor Hussin, Anis Shobirin, Hasan, Hanan, Zarei, Mohammad, Chau, De-Ming, Ramasamy, Rajesh, Saari, Nazamid
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Published: Elsevier 2023
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spelling oai:psasir.upm.edu.my:107651 http://psasir.upm.edu.my/id/eprint/107651/ Elucidating the mechanisms underlying the action of kenaf seed peptides mixture against gram-positive and gram-negative bacteria and its efficacy in whole milk preservation Arulrajah, Brisha Qoms, Mohammed S. Muhialdin, Belal J. Meor Hussin, Anis Shobirin Hasan, Hanan Zarei, Mohammad Chau, De-Ming Ramasamy, Rajesh Saari, Nazamid The present study determined the in-situ antibacterial effect of cationic kenaf seed peptides mixture (KSPM) in whole milk and further comprehensively elucidated the mechanisms underlying the bactericidal action against gram-positive and gram-negative bacteria. The application experiments in artificially contaminated milk showed that KSPM at 0.5 and 1 mg/mL displayed a stable and strong bactericidal activity within 1“6 h of treatment and lasted up to two weeks storage at room and refrigeration conditions. The in-vitro mechanistic study showed that the KSPM underwent conformational changes from random coil to ±-helix and ²-sheet in the membrane-mimetic environment. The KSPM exerted its inhibitory effect in a concentration and time dependent manner through cell membrane permeabilisation associated with membrane-disrupting effects, which is characterised by the cellular material leakage. The in-silico study revealed that the peptides efficiently interacted with bacterial peptidoglycan via hydrogen bonding, electrostatic interactions and hydrophobic forces. The peptide RALPSEHNK interacted with gram-positive and gram-negative bacterial phospholipid phosphate head group mainly through electrostatic interaction. The current work indicated that KSPM exerts its bactericidal effect by membrane-targeted mechanism and it would be a promising natural candidate to control bacterial contamination in milk and dairy-based products. Elsevier 2023-05 Article PeerReviewed Arulrajah, Brisha and Qoms, Mohammed S. and Muhialdin, Belal J. and Meor Hussin, Anis Shobirin and Hasan, Hanan and Zarei, Mohammad and Chau, De-Ming and Ramasamy, Rajesh and Saari, Nazamid (2023) Elucidating the mechanisms underlying the action of kenaf seed peptides mixture against gram-positive and gram-negative bacteria and its efficacy in whole milk preservation. LWT - Food Science and Technology, 181. art. no. 114757. ISSN 0023-6438 https://linkinghub.elsevier.com/retrieve/pii/S0023643823003365 10.1016/j.lwt.2023.114757
institution UPM IR
collection UPM IR
description The present study determined the in-situ antibacterial effect of cationic kenaf seed peptides mixture (KSPM) in whole milk and further comprehensively elucidated the mechanisms underlying the bactericidal action against gram-positive and gram-negative bacteria. The application experiments in artificially contaminated milk showed that KSPM at 0.5 and 1 mg/mL displayed a stable and strong bactericidal activity within 1“6 h of treatment and lasted up to two weeks storage at room and refrigeration conditions. The in-vitro mechanistic study showed that the KSPM underwent conformational changes from random coil to ±-helix and ²-sheet in the membrane-mimetic environment. The KSPM exerted its inhibitory effect in a concentration and time dependent manner through cell membrane permeabilisation associated with membrane-disrupting effects, which is characterised by the cellular material leakage. The in-silico study revealed that the peptides efficiently interacted with bacterial peptidoglycan via hydrogen bonding, electrostatic interactions and hydrophobic forces. The peptide RALPSEHNK interacted with gram-positive and gram-negative bacterial phospholipid phosphate head group mainly through electrostatic interaction. The current work indicated that KSPM exerts its bactericidal effect by membrane-targeted mechanism and it would be a promising natural candidate to control bacterial contamination in milk and dairy-based products.
format Article
author Arulrajah, Brisha
Qoms, Mohammed S.
Muhialdin, Belal J.
Meor Hussin, Anis Shobirin
Hasan, Hanan
Zarei, Mohammad
Chau, De-Ming
Ramasamy, Rajesh
Saari, Nazamid
spellingShingle Arulrajah, Brisha
Qoms, Mohammed S.
Muhialdin, Belal J.
Meor Hussin, Anis Shobirin
Hasan, Hanan
Zarei, Mohammad
Chau, De-Ming
Ramasamy, Rajesh
Saari, Nazamid
Elucidating the mechanisms underlying the action of kenaf seed peptides mixture against gram-positive and gram-negative bacteria and its efficacy in whole milk preservation
author_facet Arulrajah, Brisha
Qoms, Mohammed S.
Muhialdin, Belal J.
Meor Hussin, Anis Shobirin
Hasan, Hanan
Zarei, Mohammad
Chau, De-Ming
Ramasamy, Rajesh
Saari, Nazamid
author_sort Arulrajah, Brisha
title Elucidating the mechanisms underlying the action of kenaf seed peptides mixture against gram-positive and gram-negative bacteria and its efficacy in whole milk preservation
title_short Elucidating the mechanisms underlying the action of kenaf seed peptides mixture against gram-positive and gram-negative bacteria and its efficacy in whole milk preservation
title_full Elucidating the mechanisms underlying the action of kenaf seed peptides mixture against gram-positive and gram-negative bacteria and its efficacy in whole milk preservation
title_fullStr Elucidating the mechanisms underlying the action of kenaf seed peptides mixture against gram-positive and gram-negative bacteria and its efficacy in whole milk preservation
title_full_unstemmed Elucidating the mechanisms underlying the action of kenaf seed peptides mixture against gram-positive and gram-negative bacteria and its efficacy in whole milk preservation
title_sort elucidating the mechanisms underlying the action of kenaf seed peptides mixture against gram-positive and gram-negative bacteria and its efficacy in whole milk preservation
publisher Elsevier
publishDate 2023
_version_ 1819301749317435392
score 13.4562235

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