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Purification of pectinase from mango (Mangifera indica L. cv. Chokanan) waste using an aqueous organic phase system: a potential low cost source of the enzyme

As a novel method of purification, an aqueous organic phase system (AOPS) was employed to purify pectinase from mango waste. The effect of different parameters, such as the alcohol concentration (ethanol, 1-propanol, and 2-propanol), the salt type and concentration (ammonium sulfate, potassium phosp...

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Main Authors: Amid, Mehrnoush, Abdul Manap, Mohd Yazid, Mustafa, Shuhaimi
Format: Article
Jezik:English
Izdano: Elsevier 2013
Online dostop:http://psasir.upm.edu.my/id/eprint/30819/1/Purification%20of%20pectinase%20from%20mango.pdf
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id oai:psasir.upm.edu.my:30819
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spelling oai:psasir.upm.edu.my:30819 http://psasir.upm.edu.my/id/eprint/30819/ Purification of pectinase from mango (Mangifera indica L. cv. Chokanan) waste using an aqueous organic phase system: a potential low cost source of the enzyme Amid, Mehrnoush Abdul Manap, Mohd Yazid Mustafa, Shuhaimi As a novel method of purification, an aqueous organic phase system (AOPS) was employed to purify pectinase from mango waste. The effect of different parameters, such as the alcohol concentration (ethanol, 1-propanol, and 2-propanol), the salt type and concentration (ammonium sulfate, potassium phosphate and sodium citrate), the feed stock crude load, the aqueous phase pH and NaCl concentration, were investigated in the recovery of pectinase from mango peel. The partition coefficient (K), selectivity (S), purification factor (PF) and yield (Y, %) were investigated in this study as important parameters for the evaluation of enzyme recovery. The desirable partition efficiency for pectinase purification was achieved in an AOPS of 19% (w/w) ethanol and 22% (w/w) potassium phosphate in the presence of 5% (w/w) NaCl at pH 7.0. Based on the system, the purification factor of pectinase was enhanced 11.7, with a high yield of 97.1%. Elsevier 2013 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/30819/1/Purification%20of%20pectinase%20from%20mango.pdf Amid, Mehrnoush and Abdul Manap, Mohd Yazid and Mustafa, Shuhaimi (2013) Purification of pectinase from mango (Mangifera indica L. cv. Chokanan) waste using an aqueous organic phase system: a potential low cost source of the enzyme. Journal of Chromatography B, 931. pp. 17-22. ISSN 1570-0232 10.1016/j.jchromb.2013.05.009
institution UPM IR
collection UPM IR
language English
description As a novel method of purification, an aqueous organic phase system (AOPS) was employed to purify pectinase from mango waste. The effect of different parameters, such as the alcohol concentration (ethanol, 1-propanol, and 2-propanol), the salt type and concentration (ammonium sulfate, potassium phosphate and sodium citrate), the feed stock crude load, the aqueous phase pH and NaCl concentration, were investigated in the recovery of pectinase from mango peel. The partition coefficient (K), selectivity (S), purification factor (PF) and yield (Y, %) were investigated in this study as important parameters for the evaluation of enzyme recovery. The desirable partition efficiency for pectinase purification was achieved in an AOPS of 19% (w/w) ethanol and 22% (w/w) potassium phosphate in the presence of 5% (w/w) NaCl at pH 7.0. Based on the system, the purification factor of pectinase was enhanced 11.7, with a high yield of 97.1%.
format Article
author Amid, Mehrnoush
Abdul Manap, Mohd Yazid
Mustafa, Shuhaimi
spellingShingle Amid, Mehrnoush
Abdul Manap, Mohd Yazid
Mustafa, Shuhaimi
Purification of pectinase from mango (Mangifera indica L. cv. Chokanan) waste using an aqueous organic phase system: a potential low cost source of the enzyme
author_facet Amid, Mehrnoush
Abdul Manap, Mohd Yazid
Mustafa, Shuhaimi
author_sort Amid, Mehrnoush
title Purification of pectinase from mango (Mangifera indica L. cv. Chokanan) waste using an aqueous organic phase system: a potential low cost source of the enzyme
title_short Purification of pectinase from mango (Mangifera indica L. cv. Chokanan) waste using an aqueous organic phase system: a potential low cost source of the enzyme
title_full Purification of pectinase from mango (Mangifera indica L. cv. Chokanan) waste using an aqueous organic phase system: a potential low cost source of the enzyme
title_fullStr Purification of pectinase from mango (Mangifera indica L. cv. Chokanan) waste using an aqueous organic phase system: a potential low cost source of the enzyme
title_full_unstemmed Purification of pectinase from mango (Mangifera indica L. cv. Chokanan) waste using an aqueous organic phase system: a potential low cost source of the enzyme
title_sort purification of pectinase from mango (mangifera indica l. cv. chokanan) waste using an aqueous organic phase system: a potential low cost source of the enzyme
publisher Elsevier
publishDate 2013
url http://psasir.upm.edu.my/id/eprint/30819/1/Purification%20of%20pectinase%20from%20mango.pdf
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score 13.4562235

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