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Inhibition of oxidative stress and lipid peroxidation by anthocyanins from defatted Canarium odontophyllum pericarp and peel using in vitro bioassays

Canarium odontophyllum, also known as CO, is a highly nutritious fruit. Defatted parts of CO fruit are potent sources of nutraceutical. This study aimed to determine oxidative stress and lipid peroxidation effects of defatted CO pericarp and peel extracts using in vitro bioassays. Cell cytotoxic eff...

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Main Authors: Khoo, Hock Eng, Azlan, Azrina, Ismail, Amin, Abas, Faridah, Hamid, Muhajir
Format: Article
Published: Public Library of Science 2014
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spelling oai:psasir.upm.edu.my:34212 http://psasir.upm.edu.my/id/eprint/34212/ Inhibition of oxidative stress and lipid peroxidation by anthocyanins from defatted Canarium odontophyllum pericarp and peel using in vitro bioassays Khoo, Hock Eng Azlan, Azrina Ismail, Amin Abas, Faridah Hamid, Muhajir Canarium odontophyllum, also known as CO, is a highly nutritious fruit. Defatted parts of CO fruit are potent sources of nutraceutical. This study aimed to determine oxidative stress and lipid peroxidation effects of defatted CO pericarp and peel extracts using in vitro bioassays. Cell cytotoxic effect of the CO pericarp and peel extracts were also evaluated using HUVEC and Chang liver cell lines. The crude extracts of defatted CO peel and pericarp showed cytoprotective effects in t-BHP and 40% methanol-induced cell death. The crude extracts also showed no toxic effect to Chang liver cell line. Using CD36 ELISA, NAD+ and LDL inhibition assays, inhibition of oxidative stress were found higher in the crude extract of defatted CO peel compared to the pericarp extract. Hemoglobin and LDL oxidation assays revealed both crude extracts had significantly reduced lipid peroxidation as compared to control. TBARS values among defatted CO pericarp, peel, and cyanidin-3-glucoside showed no significant differences for hemoglobin and LDL oxidation assays. The protective effects of defatted CO parts, especially its peel is related to the presence of high anthocyanin that potentially offers as a pharmaceutical ingredient for cardioprotection. Public Library of Science 2014-01 Article PeerReviewed Khoo, Hock Eng and Azlan, Azrina and Ismail, Amin and Abas, Faridah and Hamid, Muhajir (2014) Inhibition of oxidative stress and lipid peroxidation by anthocyanins from defatted Canarium odontophyllum pericarp and peel using in vitro bioassays. PLOS ONE, 9 (1). art. no. e81447. pp. 1-9. ISSN 1932-6203 http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0081447 10.1371/journal.pone.0081447
institution UPM IR
collection UPM IR
description Canarium odontophyllum, also known as CO, is a highly nutritious fruit. Defatted parts of CO fruit are potent sources of nutraceutical. This study aimed to determine oxidative stress and lipid peroxidation effects of defatted CO pericarp and peel extracts using in vitro bioassays. Cell cytotoxic effect of the CO pericarp and peel extracts were also evaluated using HUVEC and Chang liver cell lines. The crude extracts of defatted CO peel and pericarp showed cytoprotective effects in t-BHP and 40% methanol-induced cell death. The crude extracts also showed no toxic effect to Chang liver cell line. Using CD36 ELISA, NAD+ and LDL inhibition assays, inhibition of oxidative stress were found higher in the crude extract of defatted CO peel compared to the pericarp extract. Hemoglobin and LDL oxidation assays revealed both crude extracts had significantly reduced lipid peroxidation as compared to control. TBARS values among defatted CO pericarp, peel, and cyanidin-3-glucoside showed no significant differences for hemoglobin and LDL oxidation assays. The protective effects of defatted CO parts, especially its peel is related to the presence of high anthocyanin that potentially offers as a pharmaceutical ingredient for cardioprotection.
format Article
author Khoo, Hock Eng
Azlan, Azrina
Ismail, Amin
Abas, Faridah
Hamid, Muhajir
spellingShingle Khoo, Hock Eng
Azlan, Azrina
Ismail, Amin
Abas, Faridah
Hamid, Muhajir
Inhibition of oxidative stress and lipid peroxidation by anthocyanins from defatted Canarium odontophyllum pericarp and peel using in vitro bioassays
author_facet Khoo, Hock Eng
Azlan, Azrina
Ismail, Amin
Abas, Faridah
Hamid, Muhajir
author_sort Khoo, Hock Eng
title Inhibition of oxidative stress and lipid peroxidation by anthocyanins from defatted Canarium odontophyllum pericarp and peel using in vitro bioassays
title_short Inhibition of oxidative stress and lipid peroxidation by anthocyanins from defatted Canarium odontophyllum pericarp and peel using in vitro bioassays
title_full Inhibition of oxidative stress and lipid peroxidation by anthocyanins from defatted Canarium odontophyllum pericarp and peel using in vitro bioassays
title_fullStr Inhibition of oxidative stress and lipid peroxidation by anthocyanins from defatted Canarium odontophyllum pericarp and peel using in vitro bioassays
title_full_unstemmed Inhibition of oxidative stress and lipid peroxidation by anthocyanins from defatted Canarium odontophyllum pericarp and peel using in vitro bioassays
title_sort inhibition of oxidative stress and lipid peroxidation by anthocyanins from defatted canarium odontophyllum pericarp and peel using in vitro bioassays
publisher Public Library of Science
publishDate 2014
_version_ 1819295665816076288
score 13.4562235

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