Soursop pectinesterases: thermostability and effect on cloud stability of soursop juice

The thermostability of two forms of purified pectinesterase from soursop fruit were studied. The heat stability data showed that PE I is more heat stable than PE II at pH 7.5. The D and Z values were evaluated in the range 45–75°C. The D values at 65°C were approximately 5.8 min and 3.3 min for PE I...

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Main Authors: Misman, Siti Arbaisah, Ali, Asbi, Abdul Hamid, Junainah, Bakar, Jamilah, Kennedy, J. F.
Format: Article
Language:English
Published: Elsevier 1997
Online Access:http://psasir.upm.edu.my/id/eprint/36028/1/Soursop%20pectinesterases%20thermostability%20and%20effect%20on%20cloud%20stability%20of%20soursop%20juice.pdf
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spelling oai:psasir.upm.edu.my:36028 http://psasir.upm.edu.my/id/eprint/36028/ Soursop pectinesterases: thermostability and effect on cloud stability of soursop juice Misman, Siti Arbaisah Ali, Asbi Abdul Hamid, Junainah Bakar, Jamilah Kennedy, J. F. The thermostability of two forms of purified pectinesterase from soursop fruit were studied. The heat stability data showed that PE I is more heat stable than PE II at pH 7.5. The D and Z values were evaluated in the range 45–75°C. The D values at 65°C were approximately 5.8 min and 3.3 min for PE I and PE II respectively. The changes in temperature required to increase the inactivation rate tenfold (Z value) were calculated at 8.5 and 8.6°C for PE I, PE II respectively. Both enzymes also tested positive for their ability to destabilise soursop juice cloud at 5 and 30°C. Cloud destabilisation by PE I occurred the fastest (large decrease in A 660 nm) in the natural juice at 30°C. Elsevier 1997-12 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/36028/1/Soursop%20pectinesterases%20thermostability%20and%20effect%20on%20cloud%20stability%20of%20soursop%20juice.pdf Misman, Siti Arbaisah and Ali, Asbi and Abdul Hamid, Junainah and Bakar, Jamilah and Kennedy, J. F. (1997) Soursop pectinesterases: thermostability and effect on cloud stability of soursop juice. Carbohydrate Polymers, 34 (3). pp. 177-182. ISSN 0144-8617; ESSN: 1879-1344 http://www.sciencedirect.com/science/article/pii/S0144861797000507 10.1016/S0144-8617(97)00050-7
institution UPM IR
collection UPM IR
language English
description The thermostability of two forms of purified pectinesterase from soursop fruit were studied. The heat stability data showed that PE I is more heat stable than PE II at pH 7.5. The D and Z values were evaluated in the range 45–75°C. The D values at 65°C were approximately 5.8 min and 3.3 min for PE I and PE II respectively. The changes in temperature required to increase the inactivation rate tenfold (Z value) were calculated at 8.5 and 8.6°C for PE I, PE II respectively. Both enzymes also tested positive for their ability to destabilise soursop juice cloud at 5 and 30°C. Cloud destabilisation by PE I occurred the fastest (large decrease in A 660 nm) in the natural juice at 30°C.
format Article
author Misman, Siti Arbaisah
Ali, Asbi
Abdul Hamid, Junainah
Bakar, Jamilah
Kennedy, J. F.
spellingShingle Misman, Siti Arbaisah
Ali, Asbi
Abdul Hamid, Junainah
Bakar, Jamilah
Kennedy, J. F.
Soursop pectinesterases: thermostability and effect on cloud stability of soursop juice
author_facet Misman, Siti Arbaisah
Ali, Asbi
Abdul Hamid, Junainah
Bakar, Jamilah
Kennedy, J. F.
author_sort Misman, Siti Arbaisah
title Soursop pectinesterases: thermostability and effect on cloud stability of soursop juice
title_short Soursop pectinesterases: thermostability and effect on cloud stability of soursop juice
title_full Soursop pectinesterases: thermostability and effect on cloud stability of soursop juice
title_fullStr Soursop pectinesterases: thermostability and effect on cloud stability of soursop juice
title_full_unstemmed Soursop pectinesterases: thermostability and effect on cloud stability of soursop juice
title_sort soursop pectinesterases: thermostability and effect on cloud stability of soursop juice
publisher Elsevier
publishDate 1997
url http://psasir.upm.edu.my/id/eprint/36028/1/Soursop%20pectinesterases%20thermostability%20and%20effect%20on%20cloud%20stability%20of%20soursop%20juice.pdf
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score 12.933938