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Molecular characterization of Escherichia coli isolated from different food sources

Escherichia coli and Escherichia coli O157 were identified from "selom" (Oenanthe stolonifera), "pegaga" (Centella asiatica), beef, chicken, lamb, buffalo, "ulam Raja" (Cosmos caudatus) and "tenggek burung" (Euodia redlevi). The bacteria were recovered using c...

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Main Authors: Cheah, Yoke Kqueen, Tay, Lee Wen, Azmi, Aida Azrina, Radu, Son, Nakaguchi, Yoshitsugu, Nishibuchi, Mitsuaki
Format: Article
Language:English
Published: Faculty of Food Science and Technology, Universiti Putra Malaysia 2015
Online Access:http://psasir.upm.edu.my/id/eprint/36106/1/%285%29.pdf
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spelling oai:psasir.upm.edu.my:36106 http://psasir.upm.edu.my/id/eprint/36106/ Molecular characterization of Escherichia coli isolated from different food sources Cheah, Yoke Kqueen Tay, Lee Wen Azmi, Aida Azrina Radu, Son Nakaguchi, Yoshitsugu Nishibuchi, Mitsuaki Escherichia coli and Escherichia coli O157 were identified from "selom" (Oenanthe stolonifera), "pegaga" (Centella asiatica), beef, chicken, lamb, buffalo, "ulam Raja" (Cosmos caudatus) and "tenggek burung" (Euodia redlevi). The bacteria were recovered using chromagenic agar. Isolated Escherichia coli and Escherichia coli 0157 were further characterized by plasmid profiling and enterobacterial repetitive intergenic consensus-polymerase chain reaction (ERIC-PCR). The virulence genes of the isolates (VT1, VT2, LT, ST, eaeA, inV) that produces pathogenic Escherichia coli and 16S rRNA gene were screened by a multiplex PCR assay. The plasmid profiling analysis showed that out of 176 isolates, only 103 isolates contained plasmids. ERIC-PCR analysis generated amplified products in the range of ~150 bp to > 1000 bp categorizing isolates into a total of 52 different profiles. Multiplex PCR showed that 20 (32.3%) of the isolates carried eaeA gene, 6 (9.7%) isolates possessed inV genes, only 1 (1.6%) have VT2 genes and 1 (1.6%) as well carried VT1 genes, 2 (3.2%) of the isolates harboured LT genes, and only 1 (1.6%) isolate possessed ST genes. There were no correlation between plasmid, ERIC-PCR and virulence genes profiles. Faculty of Food Science and Technology, Universiti Putra Malaysia 2015 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/36106/1/%285%29.pdf Cheah, Yoke Kqueen and Tay, Lee Wen and Azmi, Aida Azrina and Radu, Son and Nakaguchi, Yoshitsugu and Nishibuchi, Mitsuaki (2015) Molecular characterization of Escherichia coli isolated from different food sources. International Food Research Journal, 22 (1). pp. 31-40. ISSN 1985-4668; ESSN: 2231-7546 http://www.ifrj.upm.edu.my/22%20%2801%29%202015/%285%29.pdf
institution UPM IR
collection UPM IR
language English
description Escherichia coli and Escherichia coli O157 were identified from "selom" (Oenanthe stolonifera), "pegaga" (Centella asiatica), beef, chicken, lamb, buffalo, "ulam Raja" (Cosmos caudatus) and "tenggek burung" (Euodia redlevi). The bacteria were recovered using chromagenic agar. Isolated Escherichia coli and Escherichia coli 0157 were further characterized by plasmid profiling and enterobacterial repetitive intergenic consensus-polymerase chain reaction (ERIC-PCR). The virulence genes of the isolates (VT1, VT2, LT, ST, eaeA, inV) that produces pathogenic Escherichia coli and 16S rRNA gene were screened by a multiplex PCR assay. The plasmid profiling analysis showed that out of 176 isolates, only 103 isolates contained plasmids. ERIC-PCR analysis generated amplified products in the range of ~150 bp to > 1000 bp categorizing isolates into a total of 52 different profiles. Multiplex PCR showed that 20 (32.3%) of the isolates carried eaeA gene, 6 (9.7%) isolates possessed inV genes, only 1 (1.6%) have VT2 genes and 1 (1.6%) as well carried VT1 genes, 2 (3.2%) of the isolates harboured LT genes, and only 1 (1.6%) isolate possessed ST genes. There were no correlation between plasmid, ERIC-PCR and virulence genes profiles.
format Article
author Cheah, Yoke Kqueen
Tay, Lee Wen
Azmi, Aida Azrina
Radu, Son
Nakaguchi, Yoshitsugu
Nishibuchi, Mitsuaki
spellingShingle Cheah, Yoke Kqueen
Tay, Lee Wen
Azmi, Aida Azrina
Radu, Son
Nakaguchi, Yoshitsugu
Nishibuchi, Mitsuaki
Molecular characterization of Escherichia coli isolated from different food sources
author_facet Cheah, Yoke Kqueen
Tay, Lee Wen
Azmi, Aida Azrina
Radu, Son
Nakaguchi, Yoshitsugu
Nishibuchi, Mitsuaki
author_sort Cheah, Yoke Kqueen
title Molecular characterization of Escherichia coli isolated from different food sources
title_short Molecular characterization of Escherichia coli isolated from different food sources
title_full Molecular characterization of Escherichia coli isolated from different food sources
title_fullStr Molecular characterization of Escherichia coli isolated from different food sources
title_full_unstemmed Molecular characterization of Escherichia coli isolated from different food sources
title_sort molecular characterization of escherichia coli isolated from different food sources
publisher Faculty of Food Science and Technology, Universiti Putra Malaysia
publishDate 2015
url http://psasir.upm.edu.my/id/eprint/36106/1/%285%29.pdf
_version_ 1819295852043173888
score 13.4562235

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