γ-secretase inhibitors and modulators induce distinct conformational changes in the active sites of γ-secretase and signal peptide peptidase
γ-Secretase inhibitors (GSIs) and modulators (GSMs) are at the frontline of cancer and Alzheimer’s disease research, respectively. While both are therapeutically promising, not much is known about their interactions with proteins other than γ-secretase. Signal peptide peptidase (SPP), like γ-secreta...
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American Chemical Society
2015
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| Online Access: | http://psasir.upm.edu.my/id/eprint/46916/1/%CE%B3-secretase%20inhibitors%20and%20modulators%20induce%20distinct%20conformational%20changes%20in%20the%20active%20sites%20of%20%CE%B3-secretase%20and%20signal%20peptide%20peptidase.pdf |
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oai:psasir.upm.edu.my:46916 http://psasir.upm.edu.my/id/eprint/46916/ γ-secretase inhibitors and modulators induce distinct conformational changes in the active sites of γ-secretase and signal peptide peptidase Gertsik, Natalya Chau, De Ming Li, Yue Ming γ-Secretase inhibitors (GSIs) and modulators (GSMs) are at the frontline of cancer and Alzheimer’s disease research, respectively. While both are therapeutically promising, not much is known about their interactions with proteins other than γ-secretase. Signal peptide peptidase (SPP), like γ-secretase, is a multispan transmembrane aspartyl protease that catalyzes regulated intramembrane proteolysis. We used active site-directed photophore walking probes to study the effects of different GSIs and GSMs on the active sites of γ-secretase and SPP and found that nontransition state GSIs inhibit labeling of γ-secretase by activity-based probes but enhance labeling of SPP. The opposite is true of GSMs, which have little effect on the labeling of γ-secretase but diminish labeling of SPP. These results demonstrate that GSIs and GSMs are altering the structure of not only γ-secretase but also SPP, leading to potential changes in enzyme activity and specificity that may impact the clinical outcomes of these molecules. American Chemical Society 2015 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/46916/1/%CE%B3-secretase%20inhibitors%20and%20modulators%20induce%20distinct%20conformational%20changes%20in%20the%20active%20sites%20of%20%CE%B3-secretase%20and%20signal%20peptide%20peptidase.pdf Gertsik, Natalya and Chau, De Ming and Li, Yue Ming (2015) γ-secretase inhibitors and modulators induce distinct conformational changes in the active sites of γ-secretase and signal peptide peptidase. ACS Chemical Biology, 10 (8). pp. 1925-1931. ISSN 1554-8929; ESSN: 1554-8937 http://pubs.acs.org/doi/abs/10.1021/acschembio.5b00321 10.1021/acschembio.5b00321 |
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γ-Secretase inhibitors (GSIs) and modulators (GSMs) are at the frontline of cancer and Alzheimer’s disease research, respectively. While both are therapeutically promising, not much is known about their interactions with proteins other than γ-secretase. Signal peptide peptidase (SPP), like γ-secretase, is a multispan transmembrane aspartyl protease that catalyzes regulated intramembrane proteolysis. We used active site-directed photophore walking probes to study the effects of different GSIs and GSMs on the active sites of γ-secretase and SPP and found that nontransition state GSIs inhibit labeling of γ-secretase by activity-based probes but enhance labeling of SPP. The opposite is true of GSMs, which have little effect on the labeling of γ-secretase but diminish labeling of SPP. These results demonstrate that GSIs and GSMs are altering the structure of not only γ-secretase but also SPP, leading to potential changes in enzyme activity and specificity that may impact the clinical outcomes of these molecules. |
| format |
Article |
| author |
Gertsik, Natalya Chau, De Ming Li, Yue Ming |
| spellingShingle |
Gertsik, Natalya Chau, De Ming Li, Yue Ming γ-secretase inhibitors and modulators induce distinct conformational changes in the active sites of γ-secretase and signal peptide peptidase |
| author_facet |
Gertsik, Natalya Chau, De Ming Li, Yue Ming |
| author_sort |
Gertsik, Natalya |
| title |
γ-secretase inhibitors and modulators induce distinct conformational changes in the active sites of γ-secretase and signal peptide peptidase |
| title_short |
γ-secretase inhibitors and modulators induce distinct conformational changes in the active sites of γ-secretase and signal peptide peptidase |
| title_full |
γ-secretase inhibitors and modulators induce distinct conformational changes in the active sites of γ-secretase and signal peptide peptidase |
| title_fullStr |
γ-secretase inhibitors and modulators induce distinct conformational changes in the active sites of γ-secretase and signal peptide peptidase |
| title_full_unstemmed |
γ-secretase inhibitors and modulators induce distinct conformational changes in the active sites of γ-secretase and signal peptide peptidase |
| title_sort |
γ-secretase inhibitors and modulators induce distinct conformational changes in the active sites of γ-secretase and signal peptide peptidase |
| publisher |
American Chemical Society |
| publishDate |
2015 |
| url |
http://psasir.upm.edu.my/id/eprint/46916/1/%CE%B3-secretase%20inhibitors%20and%20modulators%20induce%20distinct%20conformational%20changes%20in%20the%20active%20sites%20of%20%CE%B3-secretase%20and%20signal%20peptide%20peptidase.pdf |
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1782722337624293376 |
| score |
12.935284 |