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Influence of high-pressure steam pretreatment on the structure of rice husk and enzymatic saccharification in a two-step system

This study aimed at developing an operational high-pressure steam pretreatment (HPSP) to effectively modify rice husk for enzymatic saccharification. The HPSP was performed at 160 to 200 °C under 0.3 to 2.8 MPa for 2 to 10 min. The efficiency of this method was based on the chemical composition, sca...

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Main Authors: Kazeem, Muinat Olanike, Md Shah, Umi Kalsom, Samsu Baharuddin, Azhari, Abdul Rahman, Nor' Aini
Format: Article
Language:English
Published: North Carolina State University 2017
Online Access:http://psasir.upm.edu.my/id/eprint/62159/1/Influence%20of%20high-pressure%20steam%20pretreatment%20on%20the%20structure%20of%20rice%20husk%20and%20enzymatic%20saccharification%20in%20a%20two-step%20system.pdf
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spelling oai:psasir.upm.edu.my:62159 http://psasir.upm.edu.my/id/eprint/62159/ Influence of high-pressure steam pretreatment on the structure of rice husk and enzymatic saccharification in a two-step system Kazeem, Muinat Olanike Md Shah, Umi Kalsom Samsu Baharuddin, Azhari Abdul Rahman, Nor' Aini This study aimed at developing an operational high-pressure steam pretreatment (HPSP) to effectively modify rice husk for enzymatic saccharification. The HPSP was performed at 160 to 200 °C under 0.3 to 2.8 MPa for 2 to 10 min. The efficiency of this method was based on the chemical composition, scanning electron microscopy (SEM), Fourier transform infrared (FTIR), and X-ray diffraction (XRD) analyses. Optimum pretreatment conditions (200 °C, 1.85 MPa for 7 min), enzyme concentration at 30 FPU/g and temperature at 60 °C for 48 h of continuous saccharification effectively produced sugar (21.1 g/L = 0.422 g/g dry substrate) at a saccharification degree of 53.87%. Conducting a second-step enzymatic saccharification resulted in additional sugar production (7.9 g/L = 0.158 g/g substrate) and a 20.44% saccharification degree. In contrast, the two-step saccharification process (48 and 24 h) achieved optimal sugar yield of 0.581 g/g substrate and saccharification degree of 73.5%. Additionally, the process improved the yield of monomeric sugars of glucose (0.465 g/g), xylose (0.010 g/g), and cellobiose (0.063 g/g). Therefore, the combination of the high-pressure steam pretreatment with thermostable cellulase from Bacillus licheniformis 2D55 in a two-step enzymatic saccharification process is an economically viable method in rice husk bioprocessing for sugar production. North Carolina State University 2017 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/62159/1/Influence%20of%20high-pressure%20steam%20pretreatment%20on%20the%20structure%20of%20rice%20husk%20and%20enzymatic%20saccharification%20in%20a%20two-step%20system.pdf Kazeem, Muinat Olanike and Md Shah, Umi Kalsom and Samsu Baharuddin, Azhari and Abdul Rahman, Nor' Aini (2017) Influence of high-pressure steam pretreatment on the structure of rice husk and enzymatic saccharification in a two-step system. BioResources, 12 (3). pp. 6207-6236. ISSN 1930-2126 http://ojs.cnr.ncsu.edu/index.php/BioRes/article/view/BioRes_12_3_6207_Kazeem_High_Pressure_Steam_Pretreatment_Rice_Husk
institution UPM IR
collection UPM IR
language English
description This study aimed at developing an operational high-pressure steam pretreatment (HPSP) to effectively modify rice husk for enzymatic saccharification. The HPSP was performed at 160 to 200 °C under 0.3 to 2.8 MPa for 2 to 10 min. The efficiency of this method was based on the chemical composition, scanning electron microscopy (SEM), Fourier transform infrared (FTIR), and X-ray diffraction (XRD) analyses. Optimum pretreatment conditions (200 °C, 1.85 MPa for 7 min), enzyme concentration at 30 FPU/g and temperature at 60 °C for 48 h of continuous saccharification effectively produced sugar (21.1 g/L = 0.422 g/g dry substrate) at a saccharification degree of 53.87%. Conducting a second-step enzymatic saccharification resulted in additional sugar production (7.9 g/L = 0.158 g/g substrate) and a 20.44% saccharification degree. In contrast, the two-step saccharification process (48 and 24 h) achieved optimal sugar yield of 0.581 g/g substrate and saccharification degree of 73.5%. Additionally, the process improved the yield of monomeric sugars of glucose (0.465 g/g), xylose (0.010 g/g), and cellobiose (0.063 g/g). Therefore, the combination of the high-pressure steam pretreatment with thermostable cellulase from Bacillus licheniformis 2D55 in a two-step enzymatic saccharification process is an economically viable method in rice husk bioprocessing for sugar production.
format Article
author Kazeem, Muinat Olanike
Md Shah, Umi Kalsom
Samsu Baharuddin, Azhari
Abdul Rahman, Nor' Aini
spellingShingle Kazeem, Muinat Olanike
Md Shah, Umi Kalsom
Samsu Baharuddin, Azhari
Abdul Rahman, Nor' Aini
Influence of high-pressure steam pretreatment on the structure of rice husk and enzymatic saccharification in a two-step system
author_facet Kazeem, Muinat Olanike
Md Shah, Umi Kalsom
Samsu Baharuddin, Azhari
Abdul Rahman, Nor' Aini
author_sort Kazeem, Muinat Olanike
title Influence of high-pressure steam pretreatment on the structure of rice husk and enzymatic saccharification in a two-step system
title_short Influence of high-pressure steam pretreatment on the structure of rice husk and enzymatic saccharification in a two-step system
title_full Influence of high-pressure steam pretreatment on the structure of rice husk and enzymatic saccharification in a two-step system
title_fullStr Influence of high-pressure steam pretreatment on the structure of rice husk and enzymatic saccharification in a two-step system
title_full_unstemmed Influence of high-pressure steam pretreatment on the structure of rice husk and enzymatic saccharification in a two-step system
title_sort influence of high-pressure steam pretreatment on the structure of rice husk and enzymatic saccharification in a two-step system
publisher North Carolina State University
publishDate 2017
url http://psasir.upm.edu.my/id/eprint/62159/1/Influence%20of%20high-pressure%20steam%20pretreatment%20on%20the%20structure%20of%20rice%20husk%20and%20enzymatic%20saccharification%20in%20a%20two-step%20system.pdf
_version_ 1819298165144158208
score 13.4562235

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