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Immobilized Talaromyces thermophilus lipase as an efficient catalyst for the production of LML-type structured lipids

LML-type structured lipids are one type of medium- and long-chain triacylglycerols. LML was synthesized using immobilized Talaromyces thermophilus lipase (TTL)-catalyzed interesterification of tricaprylin and ethyl linoleate. The resin AB-8 was chosen, and the lipase/support ratio was determined to...

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Main Authors: Lian, Weishuai, Wang, Weifei, Tan, Chin Ping, Wang, Jianrong, Wang, Yonghua
Format: Article
Language:English
Published: Springer 2019
Online Access:http://psasir.upm.edu.my/id/eprint/80148/1/Immobilized%20Talaromyces%20thermophilus%20lipase%20as%20an%20efficient%20catalyst%20for%20the%20production%20of%20LML-type%20structured%20lipids.pdf
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spelling oai:psasir.upm.edu.my:80148 http://psasir.upm.edu.my/id/eprint/80148/ Immobilized Talaromyces thermophilus lipase as an efficient catalyst for the production of LML-type structured lipids Lian, Weishuai Wang, Weifei Tan, Chin Ping Wang, Jianrong Wang, Yonghua LML-type structured lipids are one type of medium- and long-chain triacylglycerols. LML was synthesized using immobilized Talaromyces thermophilus lipase (TTL)-catalyzed interesterification of tricaprylin and ethyl linoleate. The resin AB-8 was chosen, and the lipase/support ratio was determined to be 60 mg/g. Subsequently, the immobilized TTL with strict sn-1,3 regiospecificity was applied to synthesize LML. Under the optimized conditions (60 °C, reaction time 6 h, enzyme loading of 6% of the total weight of substrates, substrate of molar ratio of ethyl linoleate to tricaprylin of 6:1), Triacylglycerols with two long- and one medium-chain FAs (DL-TAG) content as high as 52.86 mol% was obtained. Scale-up reaction further verified the industrial potential of the established process. The final product contained 85.24 mol% DL-TAG of which 97 mol% was LML after purification. The final product obtained with the high LML content would have substantial potential to be used as functional oils. Springer 2019 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/80148/1/Immobilized%20Talaromyces%20thermophilus%20lipase%20as%20an%20efficient%20catalyst%20for%20the%20production%20of%20LML-type%20structured%20lipids.pdf Lian, Weishuai and Wang, Weifei and Tan, Chin Ping and Wang, Jianrong and Wang, Yonghua (2019) Immobilized Talaromyces thermophilus lipase as an efficient catalyst for the production of LML-type structured lipids. Bioprocess and Biosystems Engineering, 42 (2). pp. 321-329. ISSN 1615-7591; ESSN: 1615-7605 https://www.researchgate.net/publication/328895530_Immobilized_Talaromyces_thermophilus_lipase_as_an_efficient_catalyst_for_the_production_of_LML-type_structured_lipids 10.1007/s00449-018-2036-7
institution UPM IR
collection UPM IR
language English
description LML-type structured lipids are one type of medium- and long-chain triacylglycerols. LML was synthesized using immobilized Talaromyces thermophilus lipase (TTL)-catalyzed interesterification of tricaprylin and ethyl linoleate. The resin AB-8 was chosen, and the lipase/support ratio was determined to be 60 mg/g. Subsequently, the immobilized TTL with strict sn-1,3 regiospecificity was applied to synthesize LML. Under the optimized conditions (60 °C, reaction time 6 h, enzyme loading of 6% of the total weight of substrates, substrate of molar ratio of ethyl linoleate to tricaprylin of 6:1), Triacylglycerols with two long- and one medium-chain FAs (DL-TAG) content as high as 52.86 mol% was obtained. Scale-up reaction further verified the industrial potential of the established process. The final product contained 85.24 mol% DL-TAG of which 97 mol% was LML after purification. The final product obtained with the high LML content would have substantial potential to be used as functional oils.
format Article
author Lian, Weishuai
Wang, Weifei
Tan, Chin Ping
Wang, Jianrong
Wang, Yonghua
spellingShingle Lian, Weishuai
Wang, Weifei
Tan, Chin Ping
Wang, Jianrong
Wang, Yonghua
Immobilized Talaromyces thermophilus lipase as an efficient catalyst for the production of LML-type structured lipids
author_facet Lian, Weishuai
Wang, Weifei
Tan, Chin Ping
Wang, Jianrong
Wang, Yonghua
author_sort Lian, Weishuai
title Immobilized Talaromyces thermophilus lipase as an efficient catalyst for the production of LML-type structured lipids
title_short Immobilized Talaromyces thermophilus lipase as an efficient catalyst for the production of LML-type structured lipids
title_full Immobilized Talaromyces thermophilus lipase as an efficient catalyst for the production of LML-type structured lipids
title_fullStr Immobilized Talaromyces thermophilus lipase as an efficient catalyst for the production of LML-type structured lipids
title_full_unstemmed Immobilized Talaromyces thermophilus lipase as an efficient catalyst for the production of LML-type structured lipids
title_sort immobilized talaromyces thermophilus lipase as an efficient catalyst for the production of lml-type structured lipids
publisher Springer
publishDate 2019
url http://psasir.upm.edu.my/id/eprint/80148/1/Immobilized%20Talaromyces%20thermophilus%20lipase%20as%20an%20efficient%20catalyst%20for%20the%20production%20of%20LML-type%20structured%20lipids.pdf
_version_ 1819299650045214720
score 13.4562235

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