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Latest advances in protein-recovery technologies from agricultural waste

In recent years, downstream bioprocessing industries are venturing into less tedious, simple, and high-efficiency separation by implementing advanced purification and extraction methods. This review discusses the separation of proteins, with the main focus on amylase as an enzyme from agricultural w...

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Main Authors: Mohd Yusree, Farhana Iylia Fatinee, Peter, Angela Paul, Mohd Nor, Mohd Zuhair, Show, Pau Loke, Mokhtar, Mohd Noriznan
Format: Article
Published: MDPI AG 2021
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spelling oai:psasir.upm.edu.my:94012 http://psasir.upm.edu.my/id/eprint/94012/ Latest advances in protein-recovery technologies from agricultural waste Mohd Yusree, Farhana Iylia Fatinee Peter, Angela Paul Mohd Nor, Mohd Zuhair Show, Pau Loke Mokhtar, Mohd Noriznan In recent years, downstream bioprocessing industries are venturing into less tedious, simple, and high-efficiency separation by implementing advanced purification and extraction methods. This review discusses the separation of proteins, with the main focus on amylase as an enzyme from agricultural waste using conventional and advanced techniques of extraction and purification via a liquid biphasic system (LBS). In comparison to other methods, such as membrane extraction, precipitation, ultrasonication, and chromatography, the LBS stands out as an efficient, cost-effective, and adaptable developing method for protein recovery. The two-phase separation method can be water-soluble polymers, or polymer and salt, or alcohol and salt, which is a simpler and lower-cost method that can be used at a larger purification scale. The comparison of different approaches in LBS for amylase purification from agricultural waste is also included. Current technology has evolved from a simple LBS into microwave-assisted LBS, liquid biphasic flotation (LBF), thermoseparation (TMP), three-phase partitioning (TPP), ultrasound-assisted LBS, and electrically assisted LBS. pH, time, temperature, and concentration are some of the significant research parameters considered in the review of advanced techniques. MDPI AG 2021-11-09 Article PeerReviewed Mohd Yusree, Farhana Iylia Fatinee and Peter, Angela Paul and Mohd Nor, Mohd Zuhair and Show, Pau Loke and Mokhtar, Mohd Noriznan (2021) Latest advances in protein-recovery technologies from agricultural waste. Foods, 10 (11). art. no. 2748. pp. 1-20. ISSN 2304-8158 https://www.mdpi.com/2304-8158/10/11/2748 10.3390/foods10112748
institution UPM IR
collection UPM IR
description In recent years, downstream bioprocessing industries are venturing into less tedious, simple, and high-efficiency separation by implementing advanced purification and extraction methods. This review discusses the separation of proteins, with the main focus on amylase as an enzyme from agricultural waste using conventional and advanced techniques of extraction and purification via a liquid biphasic system (LBS). In comparison to other methods, such as membrane extraction, precipitation, ultrasonication, and chromatography, the LBS stands out as an efficient, cost-effective, and adaptable developing method for protein recovery. The two-phase separation method can be water-soluble polymers, or polymer and salt, or alcohol and salt, which is a simpler and lower-cost method that can be used at a larger purification scale. The comparison of different approaches in LBS for amylase purification from agricultural waste is also included. Current technology has evolved from a simple LBS into microwave-assisted LBS, liquid biphasic flotation (LBF), thermoseparation (TMP), three-phase partitioning (TPP), ultrasound-assisted LBS, and electrically assisted LBS. pH, time, temperature, and concentration are some of the significant research parameters considered in the review of advanced techniques.
format Article
author Mohd Yusree, Farhana Iylia Fatinee
Peter, Angela Paul
Mohd Nor, Mohd Zuhair
Show, Pau Loke
Mokhtar, Mohd Noriznan
spellingShingle Mohd Yusree, Farhana Iylia Fatinee
Peter, Angela Paul
Mohd Nor, Mohd Zuhair
Show, Pau Loke
Mokhtar, Mohd Noriznan
Latest advances in protein-recovery technologies from agricultural waste
author_facet Mohd Yusree, Farhana Iylia Fatinee
Peter, Angela Paul
Mohd Nor, Mohd Zuhair
Show, Pau Loke
Mokhtar, Mohd Noriznan
author_sort Mohd Yusree, Farhana Iylia Fatinee
title Latest advances in protein-recovery technologies from agricultural waste
title_short Latest advances in protein-recovery technologies from agricultural waste
title_full Latest advances in protein-recovery technologies from agricultural waste
title_fullStr Latest advances in protein-recovery technologies from agricultural waste
title_full_unstemmed Latest advances in protein-recovery technologies from agricultural waste
title_sort latest advances in protein-recovery technologies from agricultural waste
publisher MDPI AG
publishDate 2021
_version_ 1782761292502663168
score 12.935284

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