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Direct analysis of anthocyanins-rich Mao fruit juice using sample dilution method based on chromophores/fluorophores of both cyanidin-3-glucoside and pelargonidin-3-glucoside

Anthocyanins are mostly classified as natural colorants in fruit, vegetables and other plant materials. The anthocyanin-rich foods or food products are of interest because of their health benefits. In Thailand, “Mao” (Antidesma thwaitesianum Muell. Arg.) the wild berry fruit with reddish violet pigm...

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Main Authors: Sripakdee, T., Mahachai, R., Chanthai, S
Format: Journal Contribution
Language:English
Published: 2018
Subjects:
Antidesma
Fruit juices
Anthocyanins
Cyanidin
Pelargonidin
Spectrophotometry
Polyphenol content
Pigments
Absorption
Fluorescence
Online Access:http://agris.upm.edu.my:8080/dspace/handle/0/14803
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spelling oai:http:--agris.upm.edu.my:0-14803Direct analysis of anthocyanins-rich Mao fruit juice using sample dilution method based on chromophores/fluorophores of both cyanidin-3-glucoside and pelargonidin-3-glucosideSripakdee, T.Mahachai, R.Chanthai, SAntidesmaFruit juicesAnthocyaninsCyanidinPelargonidinSpectrophotometryPolyphenol contentPigmentsAbsorptionFluorescenceAnthocyanins are mostly classified as natural colorants in fruit, vegetables and other plant materials. The anthocyanin-rich foods or food products are of interest because of their health benefits. In Thailand, “Mao” (Antidesma thwaitesianum Muell. Arg.) the wild berry fruit with reddish violet pigment at ripening stage are well known as high antioxidants according to anthocyanins and polyphenolics content. Total anthocyanins in some Mao juice samples determined by pH-differential method (AOAC method 2005.02) were ranged between 35.1-578 mg/L Cya-3-glu equiv. and 22.6-357 mg/L PGD-3-glu equiv. depending on their chromogenic absorption. In this study, direct analysis of the anthocyanins in an appropriate diluted juice was developed attributing for the photoextinction of chromophores/fluorophores of the two standards by UV-Visible and fluorescence spectrophotometry. Concerning on their spectral characteristics of Cya-3-glu and PGD-3-glu, both of their calibration curves were constructed resulting in similar trends of the pigments determined, but gave too high contents according to its dilution factor, compared with those of the standard method. In fact, since their dilution factor and recovery study are certainly validated, the developed methods are undoubtedly sensitive, even if, due to negligibly biased spectral interferences. Advantage of the specific extinction of any major anthocyanins in the juice samples with at least 103-fold dilution factor is, therefore, unexpectedly reported.2018-10-01T09:00:25Z2018-10-01T09:00:25Z2017Journal ContributionArticleNon-RefereedInternational Food Research Journal, 24 (1), p. 215-222ISSN (Online): 2231 7546http://agris.upm.edu.my:8080/dspace/handle/0/14803MY2018050914enhttp://www.ifrj.upm.edu.my/24%20(01)%202017/(27).pdfThailandhttp://www.oceandocs.org/license
institution AGRIS
collection AGRIS
language English
topic Antidesma
Fruit juices
Anthocyanins
Cyanidin
Pelargonidin
Spectrophotometry
Polyphenol content
Pigments
Absorption
Fluorescence
spellingShingle Antidesma
Fruit juices
Anthocyanins
Cyanidin
Pelargonidin
Spectrophotometry
Polyphenol content
Pigments
Absorption
Fluorescence
Sripakdee, T.
Mahachai, R.
Chanthai, S
Direct analysis of anthocyanins-rich Mao fruit juice using sample dilution method based on chromophores/fluorophores of both cyanidin-3-glucoside and pelargonidin-3-glucoside
description Anthocyanins are mostly classified as natural colorants in fruit, vegetables and other plant materials. The anthocyanin-rich foods or food products are of interest because of their health benefits. In Thailand, “Mao” (Antidesma thwaitesianum Muell. Arg.) the wild berry fruit with reddish violet pigment at ripening stage are well known as high antioxidants according to anthocyanins and polyphenolics content. Total anthocyanins in some Mao juice samples determined by pH-differential method (AOAC method 2005.02) were ranged between 35.1-578 mg/L Cya-3-glu equiv. and 22.6-357 mg/L PGD-3-glu equiv. depending on their chromogenic absorption. In this study, direct analysis of the anthocyanins in an appropriate diluted juice was developed attributing for the photoextinction of chromophores/fluorophores of the two standards by UV-Visible and fluorescence spectrophotometry. Concerning on their spectral characteristics of Cya-3-glu and PGD-3-glu, both of their calibration curves were constructed resulting in similar trends of the pigments determined, but gave too high contents according to its dilution factor, compared with those of the standard method. In fact, since their dilution factor and recovery study are certainly validated, the developed methods are undoubtedly sensitive, even if, due to negligibly biased spectral interferences. Advantage of the specific extinction of any major anthocyanins in the juice samples with at least 103-fold dilution factor is, therefore, unexpectedly reported.
format Journal Contribution
author Sripakdee, T.
Mahachai, R.
Chanthai, S
author_facet Sripakdee, T.
Mahachai, R.
Chanthai, S
author_sort Sripakdee, T.
title Direct analysis of anthocyanins-rich Mao fruit juice using sample dilution method based on chromophores/fluorophores of both cyanidin-3-glucoside and pelargonidin-3-glucoside
title_short Direct analysis of anthocyanins-rich Mao fruit juice using sample dilution method based on chromophores/fluorophores of both cyanidin-3-glucoside and pelargonidin-3-glucoside
title_full Direct analysis of anthocyanins-rich Mao fruit juice using sample dilution method based on chromophores/fluorophores of both cyanidin-3-glucoside and pelargonidin-3-glucoside
title_fullStr Direct analysis of anthocyanins-rich Mao fruit juice using sample dilution method based on chromophores/fluorophores of both cyanidin-3-glucoside and pelargonidin-3-glucoside
title_full_unstemmed Direct analysis of anthocyanins-rich Mao fruit juice using sample dilution method based on chromophores/fluorophores of both cyanidin-3-glucoside and pelargonidin-3-glucoside
title_sort direct analysis of anthocyanins-rich mao fruit juice using sample dilution method based on chromophores/fluorophores of both cyanidin-3-glucoside and pelargonidin-3-glucoside
publishDate 2018
url http://agris.upm.edu.my:8080/dspace/handle/0/14803
_version_ 1819285079089741824
score 13.4562235

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