Cover Image

Modification of gelatin–DNA interaction for optimised DNA extraction from gelatin and gelatin capsule

BACKGROUND: Poor quality and quantity of DNA extracted from gelatin and gelatin capsules often causes failure in the determination of animal species using PCR. Gelatin, which is mainly derived from porcine and bovine, has been a matter of concern among customers in order to fulfill religious obligat...

Full description

Saved in:
Bibliographic Details
Main Authors: Mohamad, Nurhidayatul Asma, Mustafa, Shuhaimi, El Sheikha, Aly Farag, Khairil Mokhtar, Nur Fadhilah, Ismail, Amin, Ali, Md. Eaqub
Format: Article
Language:English
Published: Society of Chemical Industry 2016
Online Access:http://psasir.upm.edu.my/id/eprint/59650/1/Modification%20of%20gelatin%E2%80%93DNA%20interaction%20for%20optimised%20DNA%20extraction%20from%20gelatin%20and%20gelatin%20capsule.pdf
Tags: Add Tag
No Tags, Be the first to tag this record!
id oai:psasir.upm.edu.my:59650
record_format eprints
spelling oai:psasir.upm.edu.my:59650 http://psasir.upm.edu.my/id/eprint/59650/ Modification of gelatin–DNA interaction for optimised DNA extraction from gelatin and gelatin capsule Mohamad, Nurhidayatul Asma Mustafa, Shuhaimi El Sheikha, Aly Farag Khairil Mokhtar, Nur Fadhilah Ismail, Amin Ali, Md. Eaqub BACKGROUND: Poor quality and quantity of DNA extracted from gelatin and gelatin capsules often causes failure in the determination of animal species using PCR. Gelatin, which is mainly derived from porcine and bovine, has been a matter of concern among customers in order to fulfill religious obligation and safety precaution against several transmissible infectious diseases associated with bovine species. Thus, optimised DNA extraction from gelatin is very important for successful real-time PCR detection of gelatin species. In this work, the DNA extraction method was optimised in terms of lysis incubation period and inclusion of pre-treatment pH modification of samples. RESULTS: The yield of DNA extracted from porcine gelatin was significantly increased when the pH of the samples was adjusted to pH 8.5 prior to DNA precipitation with isopropanol. The optimal pH for DNA precipitation from bovine gelatin solution was then determined at the original pH range of solution: pH 7.6 to 8. A DNA fragment of approximately 300 base pairs was available for PCR amplification. CONCLUSION: DNA extracted from gelatin and commercially available capsules has been successfully utilised for species detection using real-time PCR assay. However, significant adulterations of porcine and bovine in pure gelatin and capsules have been detected, which require further analytical techniques for validation. Society of Chemical Industry 2016 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/59650/1/Modification%20of%20gelatin%E2%80%93DNA%20interaction%20for%20optimised%20DNA%20extraction%20from%20gelatin%20and%20gelatin%20capsule.pdf Mohamad, Nurhidayatul Asma and Mustafa, Shuhaimi and El Sheikha, Aly Farag and Khairil Mokhtar, Nur Fadhilah and Ismail, Amin and Ali, Md. Eaqub (2016) Modification of gelatin–DNA interaction for optimised DNA extraction from gelatin and gelatin capsule. Journal of the Science of Food and Agriculture, 96 (7). pp. 2344-2351. ISSN 0022-5142; ESSN: 1097-0010 http://onlinelibrary.wiley.com/wol1/doi/10.1002/jsfa.7482/abstract 10.1002/jsfa.7482
institution UPM IR
collection UPM IR
language English
description BACKGROUND: Poor quality and quantity of DNA extracted from gelatin and gelatin capsules often causes failure in the determination of animal species using PCR. Gelatin, which is mainly derived from porcine and bovine, has been a matter of concern among customers in order to fulfill religious obligation and safety precaution against several transmissible infectious diseases associated with bovine species. Thus, optimised DNA extraction from gelatin is very important for successful real-time PCR detection of gelatin species. In this work, the DNA extraction method was optimised in terms of lysis incubation period and inclusion of pre-treatment pH modification of samples. RESULTS: The yield of DNA extracted from porcine gelatin was significantly increased when the pH of the samples was adjusted to pH 8.5 prior to DNA precipitation with isopropanol. The optimal pH for DNA precipitation from bovine gelatin solution was then determined at the original pH range of solution: pH 7.6 to 8. A DNA fragment of approximately 300 base pairs was available for PCR amplification. CONCLUSION: DNA extracted from gelatin and commercially available capsules has been successfully utilised for species detection using real-time PCR assay. However, significant adulterations of porcine and bovine in pure gelatin and capsules have been detected, which require further analytical techniques for validation.
format Article
author Mohamad, Nurhidayatul Asma
Mustafa, Shuhaimi
El Sheikha, Aly Farag
Khairil Mokhtar, Nur Fadhilah
Ismail, Amin
Ali, Md. Eaqub
spellingShingle Mohamad, Nurhidayatul Asma
Mustafa, Shuhaimi
El Sheikha, Aly Farag
Khairil Mokhtar, Nur Fadhilah
Ismail, Amin
Ali, Md. Eaqub
Modification of gelatin–DNA interaction for optimised DNA extraction from gelatin and gelatin capsule
author_facet Mohamad, Nurhidayatul Asma
Mustafa, Shuhaimi
El Sheikha, Aly Farag
Khairil Mokhtar, Nur Fadhilah
Ismail, Amin
Ali, Md. Eaqub
author_sort Mohamad, Nurhidayatul Asma
title Modification of gelatin–DNA interaction for optimised DNA extraction from gelatin and gelatin capsule
title_short Modification of gelatin–DNA interaction for optimised DNA extraction from gelatin and gelatin capsule
title_full Modification of gelatin–DNA interaction for optimised DNA extraction from gelatin and gelatin capsule
title_fullStr Modification of gelatin–DNA interaction for optimised DNA extraction from gelatin and gelatin capsule
title_full_unstemmed Modification of gelatin–DNA interaction for optimised DNA extraction from gelatin and gelatin capsule
title_sort modification of gelatin–dna interaction for optimised dna extraction from gelatin and gelatin capsule
publisher Society of Chemical Industry
publishDate 2016
url http://psasir.upm.edu.my/id/eprint/59650/1/Modification%20of%20gelatin%E2%80%93DNA%20interaction%20for%20optimised%20DNA%20extraction%20from%20gelatin%20and%20gelatin%20capsule.pdf
_version_ 1819297963259723776
score 13.4562235

Similar Items